摘要
本研究以‘津优35号’黄瓜(Cucumis sativus)幼苗为试验材料,在相同的日累积光量和光周期条件下设置稳态光照(CK)、移动光照(YD)和频闪光照(PS)3个光照处理,以期探究不同波动光环境对黄瓜幼苗生长和生理生化的影响。结果表明:与CK相比,YD和PS处理显著降低黄瓜幼苗的株高、壮苗指数、地上部分及地下部分干鲜重、根系生长、根系活力等指标,不利于壮苗的培育。YD和PS处理还会显著降低黄瓜幼苗的最大净光合速率(P_(n,max))和电子传递效率(φ_(Eo)),下调psbA、Cytb_(6)/f和psaA基因表达量,抑制植物的光合能力。与CK和YD相比,PS处理降低黄瓜幼苗表观量子效率(AQY)和叶片组织结构紧密度(CTR),升高光补偿点(LCP)和叶绿素a/b,使LHCLL基因表达量下调,单位反应中心吸收的光能(ABS/RC)降低,但相对稳定的较高光强使单位面积有活性反应中心数量(RC/CS_(o))增加。综上所述,在工厂化育苗过程中应充分考虑波动光照的低光和高光的持续时间,通过智能化装备精准调控植物生长的光照环境。
In this study,‘Jinyou 35’cucumber(Cucumis sativus)seeding was used as the experimental material,and three light treatments,including steady state light(CK),moving light(YD)and frequent flash light(PS),were set up under the same daily light integral and photoperiod,in order to investigate the effects of different fluctuating light strategies on the physiology and biochemistry of cucumber seedlings.The results show that,YD and PS treatments significantly reduced the plant height,seedling index,fresh and dry weights of shoot and root,root growth and root vigor of cucumber seedlings;compared with CK,which were not optimal to the cultivation of strong seedlings.Furthermore,YD and PS treatments also significantly reduced the maximum net photosynthetic rate(P_(n,max))and electron transfer efficiency(φ_(Eo))of cucumber seedlings,and down-regulated the expression of related genes psbA,Cytb_(6)/f and psaA,which inhibited the photosynthetic capacity of plants.Compare to CK and YD treatments,PS reduced the leaf tissue structure tightness(CTR),apparent quantum efficiency(AQY)and light energy absorbed per reaction center(ABS/RC),and down-regulated LHCLL gene expression while increased chlorophyll a/b and light compensation point(LCP),but relatively higher stable light intensity increased the number of active reaction centers per unit area(RC/CS_(o)).In summary,in the process of factory nursery we should pay more attention to the duration of high and low fluctuating light,and accurately regulate the light environment of plant growth through intelligent equipment.
作者
孙启颖
康红旗
姜玮莉
孔静雯
周璇
孙坡
刘彬彬
李清明
SUN Qiying;KANG Hongqi;JIANG Weili;KONG Jingwen;ZHOU Xuan;SUN Po;LIU Binbin;LI Qingming(Chengdu Agricultural Science and Technology Center,Chengdu 610299,China;College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China;College of Food and Biological Engineering,Chengdu University,Chengdu 610106,China;Institute of Urban Agriculture,Chinese Academy of Agricultural Sciences,Chengdu 610299,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2024年第8期1319-1328,共10页
Plant Physiology Journal
基金
国家自然科学基金(31872154和31902092)
中国农业科学院科技创新工程(34-IUA-03)
成都农业科技中心地方财政专项资金项目(NASC2020KR01和NASC2023ST06)。
关键词
黄瓜
波动光
生理适应
工厂化育苗
cucumber
fluctuating light
physiological adaptation
factory nursery
