摘要
【目的】建立石橄榄组织培养快繁技术体系。【方法】以石橄榄全株为试材,研究外植体的生长情况,筛选出最佳外植体及培养基,优化石橄榄种子萌发、壮苗增殖等培养条件。【结果】石橄榄组织培养最佳外植体为种子,其根、根茎、假鳞茎均未萌发,并出现长霉和化水的情况,而种子均可萌发。石橄榄种子萌发启动最佳培养基配方为1/2MS+6-BA 1.5 mg/L+NAA 0.8 mg/L+6-KT 0.1 mg/L,此条件下石橄榄原球茎变绿色。在基础培养基中分别加入香蕉泥(50 g/L)、土豆泥(50 g/L)、椰汁(50 mL/L)进行壮苗有机物筛选发现,添加香蕉泥的培养基中石橄榄整体长势良好、均一,假鳞茎多,株高与叶长均高于培养基添加土豆泥或椰汁的石橄榄,最终选出50 g/L香蕉泥作为最佳添加有机物加入后续试验的培养基中。石橄榄壮苗优化的最佳培养配方为1/2MS+50 g/L香蕉泥+6-BA 0.5 mg/L+NAA 0.5 mg/L+6-KT 0.3 mg/L,此条件下石橄榄长势好、株高较高。石橄榄壮苗生根的最佳培养基配方为MS+50 g/L香蕉泥+IBA 0.5 mg/L+NAA 0.5 mg/L,此条件下石橄榄假鳞茎多、气生根多立于苗上下。【结论】建立适合石橄榄种子组织培养快繁技术体系,石橄榄种子最佳萌发启动培养基配方为1/2MS+6-BA 1.5 mg/L+NAA 0.8 mg/L+6-KT 0.1 mg/L;最佳壮苗优化培养基配方是1/2MS+50 g/L香蕉泥+6-BA 0.5 mg/L+NAA 0.5 mg/L+6-KT 0.3 mg/L;最佳生根培养基配方是MS+50 g/L香蕉泥+IBA 0.5 mg/L+NAA 0.5 mg/L,为石橄榄的资源保护与利用及其规模化生产奠定基础。
【Objective】The study was carried out to establish a technical system suitable for tissue culture and rapid propagation of Pholidota chinensis Lindl.【Method】With the whole plant of P.chinensis Lindl as test material,the growth of explants was studied,the best explant and culture medium were selected,and the culture conditions such as seed germination and its roots,rhizomes and pseudobulbs did not germinate,but the seeds could germinate.The optimum culture formula of P.chinensis Lindl.was 1/2MS+6-BA 1.5 mg/L+NAA 0.8 mg/L+6-KT 0.1 mg/L and the protocorm of P.chinensis Lindl.turned green under this condition.The organic matter selection of strong se edlings was conducted by adding banana puree(50 g/L),mashed potato(50 g/L)and coconut water(50 mL/L)to the basal medium,and it was found that the overall growth of P.chinensis Lindl.in the medium with banana puree was good and uniform with many pseudobulbs,and the plant height and leaf length were better than those of the P.chinensis Lindl.with mashed potato or coconut water added to the medium.Finally,50 g/L banana puree was selected as the best organic matter added to the medium in the subsequent experiment.The best culture formula for the optimization of strong seedlings was 1/2MS+50 g/L banana puree+6-BA 0.5 mg/L+NAA 0.5 mg/L+6-KT 0.3 mg/L.Under this condition,the P.chinensis Lindl.grew well and the plant height was better.The best medium formula for rooting of strong seedlings was MS+50 g/L banana puree+IBA 0.5 mg/L+NAA 0.5 mg/L.Under this condition,there were more pseudobulbs and more aerial roots on the seedlings.【Conclusion】To establish a technical system suitable for tissue culture and rapid propagation of P.chinensis Lindl.seeds,the best formula for the germination culture of P.chinensis Lindl.seeds is 1/2MS+6-BA 1.5 mg/L+NAA 0.8 mg/L+6-KT 0.1 mg/L;the optimum culture formula for the optimization of strong seedlings is 1/2MS+50 g/L banana puree+6-BA 0.5 mg/L+NAA 0.5 mg/L+6-KT 0.3 mg/L;the best medium formula for rooting is MS+50 g/L banana puree+IBA 0.5 mg/L+NAA 0.5 mg/L.The study results lay a foundation for the resource conservation and utilization of P.chinensis Lindl.and its large-scale production.
作者
朱华
吴焕
宋巧玲
张淼
陈龙
黄诗萍
梁仁涛
吴文源
ZHU Hua;WU Huan;SONG Qiaoling;ZHANG Miao;CHEN Long;HUANG Shiping;LIANG Rentao;WU Wenyuan(Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine/Guangxi Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine/Guangxi Zhuang Autonomous Region Ethnic Medicine Resources and Application Research Center of Engineering,Nanning 530200,China)
出处
《广东农业科学》
CAS
2024年第8期71-79,共9页
Guangdong Agricultural Sciences
基金
广西高校中青年教师科研基础能力提升项目(2024KY0310)
广西一流学科中药学(民族药学)项目(桂教科研[2018]12号)
广西中医药重点学科壮药学项目(GZXK-Z-20-64)
广西壮瑶药重点实验室项目(桂科基字[2014]32号)。
关键词
石橄榄
组织培养
培养配方
种子萌发
壮苗
生根
Pholidota chinensis Lindl.
tissue culture
culture formula
seed germination
strong seedlings
rooting
