摘要
研究旨在探讨菌酶协同发酵羽毛粉生产蛋白饲料的可行性,并确定生产工艺参数。将实验室筛选得到的角蛋白高效降解菌株与市售角蛋白酶结合,协同固态发酵羽毛粉。以体外胃蛋白酶消化率为考察指标,对发酵时间、接种量、菌酶比例、加水量进行单因素和正交试验分析。结果显示,最佳发酵工艺为羽毛粉加水量45%、菌酶比例1∶1、接种量6%、发酵时间7 d。发酵后羽毛粉的体外胃蛋白酶消化率为63.97%,较发酵前提高了15.22%(P<0.01);可溶性蛋白含量为3.51 g/L,较发酵前提高了80%(P<0.05)。研究表明,在最佳工艺参数下,利用菌酶协同固态发酵羽毛粉可以高效生产蛋白饲料,提高了羽毛粉的饲用价值。
The study explored the feasibility of producing protein feed by microbial enzyme synergy fermentation of feather meal and to determine the process parameters for this production.The keratin-degrading bacterial strains selected from the laboratory were combined with commercially available keratinase enzyme preparations to utilize microbial enzyme synergy in solid-state fermentation of feather meal.The in vitro pepsin digestibility was used as the index to analyze the fermentation time,inoculation amount,microbial enzyme ratio,and water addition through single-factor and orthogonal test analysis.The results showed that the optimal fermentation process was obtained:The water addition to feather meal was 45%,the microbial enzyme ratio was 1∶1,the inoculation amount was 6%,and the fermentation time was 7 days.After fermentation,the in vitro pepsin digestibility of feather meal was 63.97%,which was significantly improved by 15.22%compared to before fermentation(P<0.01),and the soluble protein content was 3.51 g/L,which was significantly increased by 80%compared to before fermentation(P<0.05).The study indicates that under the optimal process parameters,using microbial enzyme synergy in solid-state fermentation of feather meal can efficiently produce protein feed and enhance the feed value of feather meal.
作者
贾小平
董宝花
龚建刚
刘芳宇
何俊鑫
王琪
冯志华
张海华
JIA Xiao-ping;DONG Bao-hua;GONG Jian-gang;LIU Fang-yu;HE Jun-xin;WANG Qi;FENG Zhi-hua;ZHANG Hai-hua
出处
《饲料研究》
CAS
北大核心
2024年第17期84-88,共5页
Feed Research
基金
河北农业大学2022—2023大学生创新创业训练计划项目(项目编号:2022047)
河北省现代农业产业技术体系蛋鸡肉鸡(家禽)营养与饲料(项目编号:HBCT2023210202)
河北省重点研发计划项目乡村振兴技术创新专项(项目编号22327308D)。
关键词
羽毛粉
菌酶协同
固态发酵
蛋白饲料
feather meal
microbial enzyme synergy
solid-state fermentation
protein feed
