摘要
为实现淫羊藿部位的鉴别和质量控制,对伏牛山阴坡和阳坡的野生淫羊藿不同部位红外光谱的差异进行了分析比对。将采集的野生淫羊藿样品分为根、须根、茎、叶4部分,阴干,粉碎,过筛后,利用傅里叶变换红外光谱仪获得原始红外光谱,平滑降噪,基线校正,归一化处理后求导获得其二阶导数红外光谱。比对了阴坡和阳坡野生淫羊藿4个部位红外光谱的差异,并结合小气候特征分析差异形成的原因。结果显示:伏牛山野生淫羊藿4个部位的原始和二阶导数红外光谱均存在差异。对于二阶导数红外光谱,根在1466,1452 cm^(-1)以及须根在1466,1453 cm^(-1)处有双峰,根在938 cm^(-1)以及须根937 cm^(-1)处有单峰,可以与茎、叶进行区分;须根在1260 cm^(-1)处的吸收峰强而锐,可与根进行区分;茎在1648 cm^(-1)处有宽峰,可以与其他部位进行区分;叶在1440 cm^(-1)处有明显吸收峰,可以与其他部位进行区分。阳坡和阴坡野生淫羊藿所含化学成分整体相似,但也存在差异。阳坡淫羊藿物质积累量较阴坡的多,尤其是根和茎,可能与两个地区的光照时长和光照强度不同有关。
To realise the identification and quality control of parts of Epimedium brevicornuin Maxim.,the infrared spectra difference of different parts of wild Epimedium brevicornuin Maxim.from northern and southern slopes of Funiu Mountain was analyzed and compared.The collected wild Epimedium brevicornuin Maxim.samples were divided into 4 parts of roots,fibrous roots,stems,and leaves.After drying in the shade,crushing,and sieving,the raw infrared spectra were obtained by Fourier transform infrared spectrometer.After smoothing noise reduction,baseline correction,normalization,and derivative,the second-order derivative infrared spectra were obtained.The differences in infrared spectra between the 4 parts of wild Epimedium brevicornuin Maxim.from northern and southern slopes were compared,and the reason of the differences was analyzed in combination with the characteristics of the microclimate.It was shown that there were differences in raw infrared spectra and second-order derivative infrared spectra of the 4 parts of wild Epimedium brevicornuin Maxim.from Funiu Mountain.For secondorder derivative infrared spectra,the roots and fibrous roots of Epimedium brevicornuin Maxim.had double peaks at 1466,1452 cm^(−1) and 1466,1453 cm^(−1),and single peaks at 938 cm^(−1) and 937 cm^(−1),which could be differentiated from stems and leaves.Fibrous roots had a strong and sharp absorption peak at 1260 cm^(−1),which could be differentiated from roots.Stem had a strong and sharp peak at 1648 cm^(−1),which could be differentiated from the other parts.Leaves had an obvious absorption peak at 1440 cm^(−1),which could be differentiated from the other parts.The chemical components in Epimedium brevicornuin Maxim.from northern and southern slopes of mountains were similar as a whole,but there were also differences.Epimedium brevicornuin Maxim.from northern slopes of mountain accumulated more substances than that from southern slopes of mountain,especially in roots and stems,which might be related to the difference in illumination duration and illumination intensity of the 2 regions.
作者
裴莉昕
何江龙
纪宝玉
杨林林
陈随清
李盼盼
李秀清
张琼琼
PEI Lixin;HE Jianglong;JI Baoyu;YANG Linlin;CHEN Suiqing;LI Panpan;LI Xiuqing;ZHANG Qiongqiong(School of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Henan Key Laboratory of TCM Resources and Chemistry,Zhengzhou 450046,China;Henan Provincial Herbal Ecological Planting Engineering Technology Research Center,Zhengzhou 450046,China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2024年第10期1047-1054,共8页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
河南省重点研发与推广专项科技公关(242102311260)
国家药品监督管理局重点实验室开放课题项目(KF202105)。
关键词
伏牛山
野生淫羊藿
傅里叶变换红外光谱法
药用部位
区分
物质积累
生长环境
Funiu Mountain
wild Epimedium brevicornuin Maxim.
Fourier transform infrared spectrometry
medicinal part
distinguishment
material accumulation
growing environment
