摘要
目的检测胃癌患者外周血清中长链非编码RNA核富集转录本(NEAT)1的相对表达水平,探讨其应用于胃癌辅助诊断的可能性。方法选取2017年11月至2019年12月南通市肿瘤医院收治的胃癌初诊患者101例为胃癌组,胃良性病变患者60例为胃良性病变组,以及同期体检健康者76例为对照组。分别检测各组血清标本中lncRNA NEAT1相对表达水平和癌胚抗原(CEA)、糖类抗原19-9(CA19-9)水平,分析lncRNA NEAT1与临床病理参数的相关性,并用受试者工作特征(ROC)曲线评价lncRNA NEAT1、CEA、CA19-9单独及联合应用时的诊断效能。结果胃癌组、胃良性病变组及对照组血清中lncRNA NEAT1的相对表达水平分别为2.163(1.357,2.843)、1.176(0.677,1.381)、1.063(0.570,1.308),组间比较差异有统计学意义(H=52.467,P<0.001)。胃癌患者血清lncRNA NEAT1相对表达水平与患者性别(P=0.353)、年龄(P=0.382)无关,而与肿瘤最大径(P=0.031)、TNM分期(P=0.037)及淋巴结转移(P=0.046)有关。胃癌患者血清中lncRNA NEAT1相对表达水平与CEA、CA19-9均无相关性(P>0.05)。ROC曲线结果显示,lncRNA NEAT1诊断胃癌的曲线下面积和灵敏度均高于CEA、CA19-9单独诊断,三者联合诊断时效能最高。结论胃癌患者外周血lncRNA NEAT1相对表达水平升高,且与部分临床病理参数相关,lncRNA NEAT1有望成为胃癌辅助诊断新的标志物。
Objective To detect the relative expression level of long non-coding RNA nuclear enriched abundant transcript(NEAT)1 in peripheral serum of patients with gastric cancer,and to explore the possibility of its application in the auxiliary diagnosis of gastric cancer.Methods A total of 101 patients with newly diagnosed gastric cancer in Nantong Tumor Hospital from November 2017 to December 2019 were selected as the gastric cancer group,60 patients with benign gastric lesions were selected as the gastric benign lesions group,and 76 healthy people were selected as the control group.The relative expression levels of lncRNA NEAT1,carcinoembryonic antigen(CEA)and carbohydrate antigen 19-9(CA19-9)in serum samples of each group were detected,and the correlation between lncRNA NEAT1 and clinicopathological parameters was analyzed.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficacy of lncRNA NEAT1,CEA and CA19-9 alone or in combination.Results The relative expression levels of lncRNA NEAT1 in serum of gastric cancer group,gastric benign disease group and control group were 2.163(1.357,2.843),1.176(0.677,1.381)and 1.063(0.570,1.308),respectively,and the difference was statistically significant(H=52.467,P<0.001).The relative expression level of serum lncRNA NEAT1 in gastric cancer patients was not related to gender(P=0.353)and age(P=0.382),but was related to tumor size(P=0.031),TNM stage(P=0.037)and lymph node metastasis(P=0.046).The relative expression level of lncRNA NEAT1 in serum of gastric cancer patients was not correlated with CEA and CA19-9(P>0.05).The ROC curve showed that the area under the curve and sensitivity of lncRNA NEAT1 in the diagnosis of gastric cancer were higher than those of CEA and CA19-9.The combined diagnosis of the three had the highest diagnost ic efficiency.Conclusion The relative expression of lncRNA NEAT1 in peripheral blood of patients with gastric cancer is increased,and it is correlated with some clinicopathological parameters.lncRNA NEAT1 may be a new marker for auxiliary diagnosis of gastric cancer.
作者
张敏
朱慧婧
张小霞
顾益凤
鞠少卿
ZHANG Min;ZHU Huijing;ZHANG Xiaoxia;GU Yifeng;JU Shaoqing(Department of Clinical Laboratory,Nantong Tumor Hospital,Nantong,Jiangsu 226361,China;Department of Clinical Laboratory,Affiliated Hospital of Nantong University,Nantong,Jiangsu 226006,China)
出处
《国际检验医学杂志》
CAS
2024年第20期2433-2436,2442,共5页
International Journal of Laboratory Medicine
基金
国家自然科学基金项目(81271920,81871720)。
关键词
胃癌
长链非编码RNA核富集转录本1
辅助诊断
gastric cancer
long non-coding RNA nuclear enriched abundant transcript 1
auxiliary diagnosis
