摘要
GATA转录因子在调控植物病原真菌菌丝生长、产孢、孢子萌发和致病性等过程中具有重要作用。为探究GATA转录因子在假禾谷镰孢致病过程中的功能,基于同源检索和蛋白质结构域鉴定方法,在假禾谷镰孢基因组中鉴定到7个GATA转录因子编码基因,分别命名为FpGATA1~FpGATA7。FpGATA基因长度为614~3155 bp,含有1~3个内含子。FpGATA蛋白均含有保守的GATA结构域,等电点显示FpGATA多为碱性。采用实时荧光定量PCR分析FpGATA在假禾谷镰孢不同侵染阶段的相对表达量,发现FpGATA1、FpGATA4、FpGATA5、FpGATA6和FpGATA7在侵染早期诱导表达,FpGATA2在侵染后期表达量升高。利用真菌遗传转化方法获得假禾谷镰孢FpGATA5缺失的突变体,表型分析发现,与野生型相比,FpGATA5缺失突变体的菌丝生长速率和分生孢子产量均明显下降,接种大麦叶片和小麦胚芽鞘的致病力显著降低。综合上述结果,多个FpGATA基因的表达响应假禾谷镰孢的致病过程,且FpGATA5参与病原菌的生长、产孢和致病。
GATA transcription factors play important roles in regulating hyphal growth,conidial production,conidial germination and virulence in phytopathogenic fungi.In order to explore the function of GATA transcription factors in Fusarium pseudograminearum,a total of seven GATA transcription factors encoding genes,which were subsequently named as FpGATA1—FpGATA7,were identified from the genome of F.pseudograminearum using homology searching and domain identification.The full-length sequences of FpGATA genes were 614—3155 bp,containing one,two or three introns.All FpGATA proteins contained at least one conserved GATA domain,and most of these proteins were alkaline.The quantitative Real-time PCR was used to assay the relative expression levels of FpGATA genes during different infection stages.We found that FpGATA1,FpGATA4,FpGATA5,FpGATA6 and FpGATA7 were induced during the early infection stages,and FpGATA2 was induced in the later infection stage.The FpGATA5 was deleted in F.pseudograminearum by genetic transformation.Compared to the wild-type,the FpGATA5 deletion mutants showed a decrease in hyphal growth and conidial production,and the significantly reduced pathogenicity was observed by barely leaves and wheat coleoptiles inoculation.Overall,the results suggested that the expression of several FpGATA genes was induced during F.pseudograminearum infection,and FpGATA5 played roles in growth,conidiation and pathogenesis of F.pseudograminearum.
作者
张时雨
赵培怡
刘敏
朱文亭
彭梦雅
施艳
杨雪
李洪连
陈琳琳
ZHANG Shiyu;ZHAO Peiyi;LIU Min;ZHU Wenting;PENG Mengya;SHI Yan;YANG Xue;LI Honglian;CHEN Linlin(College of Plant Protection,Henan Agricultural University,Co-construction State Key Laboratory of Wheat and Maize Crop Science,Zhengzhou 450046,China)
出处
《华北农学报》
CSCD
北大核心
2024年第5期204-211,共8页
Acta Agriculturae Boreali-Sinica
基金
河南省重大科技专项(221100110100)
国家自然科学基金国际联合基金项目(31961143018)。
关键词
小麦茎基腐病
假禾谷镰孢
GATA转录因子
基因表达
致病力
Fusarium crown rot
Fusarium pseudograminearum
GATA transcription factor
Gene expression
Pathogenicity
