摘要
目的:建立超高效液相色谱-四极杆/飞行时间质谱法测定含五味子保健食品中的五味子甲素、五味子乙素和五味子醇甲的含量。方法:样品以70%乙腈水溶液提取后,盐析分层,合并后的乙腈层用80 mg C18固相吸附剂净化。在优化的仪器条件下,采用ESI电离源以正离子模式进行扫描,APCI电离源进行校正。结果:3种木脂素化合物在50~2000μg·L^(-1)时线性关系良好,相关系数均≥0.9990,平均回收率为87.0%~104.1%,相对标准偏差为2.98%~6.56%,检出限为1.0 mg·kg^(-1),定量限为2.5 mg·kg^(-1)。结论:该方法的前处理步骤简单、耗材少,回收率、灵敏度、选择性和重现性较高,可用于快速测定含五味子保健食品中木脂素的含量。
Objective:To establish an ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry method for simultaneous determination of schisandrin,schisandrin B and schisandrin A in health food containing Schisandra chinensis.Method:Samples were extracted with 70%acetonitrile solution,salted out and stratified,and the combined acetonitrile layer was purified with 80 mg C18 solid phase adsorbent.Under optimized instrument conditions,ESI ionization source was used for scanning in positive ion mode,and APCI ionization source was used for correction.Result:The linear relationship between the three lignans was good in the range of 50~2000μg·L^(-1),the correlation coefficients were all≥0.9990,the average recoveries were 87.0%~104.1%,the relative standard deviations were 2.98%~6.56%,the limits of detection were 1.0 mg·kg^(-1),and the limits of quantification were 2.5 mg·kg^(-1).Conclusion:The method has simple pretreatment steps,less consumables,high recovery,sensitivity,selectivity and reproducibility,and can be used for rapid determination of lignan content in health food containing Schisandra chinensis.
作者
全海倩
何明
黄水林
QUAN Haiqian;HE Ming;HUANG Shuilin(Shenzhen Shum Yip Space Food&Environmental Testing Technology Co.,Ltd.,Shenzhen 518040,China)
出处
《现代食品》
2024年第17期207-210,214,共5页
Modern Food
关键词
超高效液相色谱-四极杆/飞行时间质谱法
五味子
木脂素
ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry
Schisandra chinensis
lignans
