摘要
论文旨在研究锦鲤(Cyprinus carpio var. koi)GNPTAB(alpha/beta subunits of N-acetylglucosamine-1-phosphotransferase)基因的分子生物学特征及对维氏气单胞菌感染的应答规律。对锦鲤GNPTAB基因CDS区进行生物信息学分析,利用实时荧光定量PCR技术研究GNPTAB基因的组织分布和维氏气单胞菌(Aeromonas veronii)感染机体后的时序表达规律。结果显示,GNPTAB基因CDS区片段长度为3 747 bp,推测编码1 248个氨基酸,含有4个结构域。实时荧光定量PCR分析显示,GNPTAB基因在健康锦鲤各组织中均有表达,在肝脏表达量最高,其次是肌肉、肠道、脾脏、皮肤、中肾、头肾。维氏气单胞菌人工感染锦鲤6~72 h,GNPTAB基因在肠道组织主要呈现上调表达,在肝脏、头肾、脾脏、皮肤、肌肉组织中主要呈现下调表达。维氏气单胞菌感染锦鲤后,GNPTAB基因在这些组织中出现表达差异,推测GNPTAB参与了机体的病理生理过程或免疫应答反应。
This study aimed at exploring the molecular characteristics of GNPTAB(alpha/beta subunits of N-acetylglucosamine-1-phosphotransferase)gene and its immune response to infection with Aeromonas veronii in koi carp Cyprinus carpio var.koi.The CDS region of GNPTAB gene in koi carp was analyzed by bioinformatics.Real-time fluorescence quantitative PCR technology was applied to investigate the tissue distribution of GNPTAB gene from healthy C.carpio var.koi and its temporal expression in different tissues of the fish following challenging with A.veronii.The results showed that the length of GNPTAB gene CDS fragment was 3747 bp,which encoded 1248 amino acid and contained four specific domains.Real-time fluorescence Quantitative PCR analysis showed that GNPTAB was expressed in all tested tissues of healthy C.carpio var.koi,with the highest expression in the liver,followed by the muscle,intestine,spleen,skin,midkidney and head kidney.During 6-72 h after experimental infection with A.veronii in the C.carpio var.koi,the expression of GNPTAB was up-regulated in the intestine,while down-regulated in the liver,head kidney,spleen,skin and muscle.The differentiated expression in the tested tissues following infection with A.veronii in the C.carpio var.koi suggested that GNPTAB was involved in the pathophysiological process or the immune response against A.veronii.
作者
温思怡
韩卓然
孙敬锋
Wen Siyi;Han Zhuoran;Sun Jingfeng(College of Fisheries,Tianjin Agricultural University,Tianjin 300392,China)
出处
《天津农学院学报》
CAS
2024年第5期52-58,共7页
Journal of Tianjin Agricultural University
基金
天津市海水养殖产业技术体系创新团队项目(ITTMRS2021007)
天津市自然科学基金项目(23JCQNJC00490)。
