摘要
目的探讨癌组织微小RNA-218(miR-218)联合组蛋白伴侣抗沉默功能蛋白1B(ASF1B)预测前列腺癌根治术后患者复发的价值。方法前瞻性选取2021年1月至2023年5月新乡医学院第一附属医院收治的252例前列腺癌患者作为研究对象,所有患者均实施前列腺癌根治术,术后接受为期1年的随访,根据术后1年复发情况分为复发组50例和未复发组202例。比较癌组织、癌旁组织及两组患者癌组织miR-218、ASF1B m RNA表达水平。采用多因素Logistic回归分析癌组织miR-218、ASF1B m RNA对术后复发风险的影响;采用受试者工作特征(ROC)曲线分析癌组织miR-218、ASF1B m RNA表达水平联合预测术后复发风险的价值。结果癌组织miR-218表达水平为0.47±0.13,明显低于癌旁组织的0.86±0.21,而癌组织ASF1B mRNA表达水平为1.54±0.49,明显高于癌旁组织的1.05±0.18,差异均有统计学意义(P<0.05);复发组患者癌组织的miR-218表达水平为0.25±0.08,明显低于未复发组的0.52±0.17,而ASF1B m RNA表达水平为2.03±0.66,明显高于未复发组的1.42±0.46,差异均有统计学意义(P<0.05);经多因素Logistic回归分析结果显示,校正了术后病理分期、术前PSA、术后切缘阳性后,癌组织miR-218、ASF1B mRNA仍是术后复发的独立相关影响因素(P<0.05);经ROC分析结果显示,癌组织miR-218、ASF1B m RNA及联合检测预测术后复发的ROC曲线下面积(AUC)为0.803、0.824、0.936,联合检测的AUC大于miR-218、ASF1B mRNA,差异均有统计学意义(P<0.05)。结论癌组织miR-218、ASF1B m RNA表达与前列腺癌根治术后复发风险独立相关,联合检测抑癌因子miR-218和促癌因子ASF1B m RNA能提高对患者术后复发的预测价值,为前列腺癌根治术后分层管理、治疗决策等提供重要的参考依据。
Objective To explore the value of microRNA-218(miR-218)combined with histone chaperone anti-silencing function protein 1B(ASF1B)in predicting the recurrence of prostate cancer after radical prostatectomy.Methods A prospective study was conducted on 252 patients with prostate cancer admitted to the First Affiliated Hospital of Xinxiang Medical University from January 2021 to May 2023.All patients underwent radical prostatectomy and were followed up for one year.They were divided into a recurrence group(50 cases)and a non-recurrence group(202 cases)according to the recurrence status one year after surgery.The expression levels of miR-218 and ASF1B mRNA in cancer tissues,adjacent tissues,and cancer tissues of two groups were compared.The impact of miR-218 and ASF1B mRNA in cancer tissues on the risk of postoperative recurrence was analyzed using multivariate logistic regression.Receiver operating characteristic(ROC)curve was used to evaluate the combined predictive value of miR-218 and ASF1B mRNA expression levels in cancer tissues for predicting the risk of postoperative recurrence.Results The expression level of miR-218 in cancer tissues was 0.47±0.13,which was significantly lower than 0.86±0.21 in the adjacent tissues(P<0.05).The expression level of ASF1B mRNA in cancer tissues was 1.54±0.49,which was significantly higher than 1.05±0.18 in the paracancerous tissue(P<0.05).The expression level of miR-218 in the cancer tissue of the recurrence group was 0.25±0.08,which was significantly lower than 0.52±0.17 of the non-recurrence group(P<0.05).The expression level of ASF1B mRNA in the cancer tissues of the recurrence group was 2.03±0.66,which was significantly higher than 1.42±0.46 of the non-recurrence group(P<0.05).The results of the multivariate logistic regression analysis showed that after adjusting for postoperative pathological stage,preoperative PSA,and positive surgical margins,miR-218,and ASF1B mRNA in cancer tissues were still independent factors associated with postoperative recurrence(P<0.05).The ROC analysis results showed that the area under the ROC curve(AUC)of miR-218 and ASF1B mRNA in cancer tissues alone and in combination for predicting postoperative recurrence was 0.803,0.824,and 0.936,respectively.The AUC of combined detection was significantly greater than that of miR-218 and ASF1B mRNA(P<0.05).Conclusion The mRNA expression levels of miR-218 and ASF1B in cancer tissue are independently correlated with the risk of recurrence after radical prostatectomy.The combined detection of tumor suppressive factor miR-218 and tumor promoting factor ASF1B mRNA can improve the predictive value of postoperative recurrence,and provide an important reference for hierarchical management and treatment decision-making after radical prostatectomy.
作者
韩广业
马阔
宋伟航
刘沛
张少华
余沁楠
HAN Guang-ye;MA Kuo;SONG Wei-hang;LIU Pei;ZHANG Shao-hua;YU Qin-nan(Department of Urology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,Henan,CHINA)
出处
《海南医学》
CAS
2024年第20期2969-2973,共5页
Hainan Medical Journal
基金
河南省医学科技攻关计划联合共建项目(编号:LHGJ20220607)。
