摘要
【目的】含有束状蛋白(fasiclin)结构域的类成束状阿拉伯半乳糖蛋白(fasciclin-like arabinogalactan proteins,FLAs)在巨桉细胞壁的次生生长和合成中起着重要作用。为了获得更多有价值的信息并探索其功能,对巨桉FLA基因家族的全基因组进行分析。【方法】以巨桉全基因组数据和转录组数据为基础,通过Expasy、MEME、MEGA7.0、TBtools等生物信息学工具对EgrFLAs基因家族的核酸序列的特征、基因结构、蛋白质理化性质、蛋白保守结构域、家族系统进化关系和表达量等进行分析和预测。【结果】巨桉共有21个EgrFLAs基因,其中包括3个新发现的EgrFLAs基因,这些基因不均匀地分布在巨桉11条染色体中的9条上。所有鉴定到的EgrFLAs基因均含有束状蛋白结构域,聚为4个群组,其中D组包含最多的基因成员。在EgrFLAs基因启动子中发现了大量的顺式元件,包括发育相关、水杨酸、茉莉酸甲酯等相关响应元件。不同组织的表达谱分析表明除EgrFLA1、EgrFLA2和EgrFLA3外,聚在A组的EgrFLA5和EgrFLA7也参与了次生生长。在巨桉不定根形成过程中,EgrFLA1和EgrFLA3通过表达量逐渐增加的方式参与诱导不定根形成。经SA和MeJA处理后,EgrFLA1和EgrFLA2的表达量发生显著变化,表明EgrFLA1和EgrFLA2在巨桉的发育和胁迫反应中可能具有多种作用。【结论】在巨桉中鉴定FLA基因21个,包括新发现的3个基因。在EgrFLAs基因启动子中发现了大量发育和激素相关的顺式元件。EgrFLA1、EgrFLA2、EgrFLA3、EgrFLA5和EgrFLA7作为巨桉次生生长发育中的候选基因,将为进一步的巨桉分子育种提供宝贵的资源。
【Objective】The fasciclin-like arabinogalactan proteins(FLAs)known as containing fasciclin(FAS)domains,were identified as important roles in secondary growth and synthesis of the cell wall in eucalyptus.A whole genome study of the FLA gene family of Eucalyptus grandis was performed in this study to gain more valuable information and explore their function.【Method】Basing on the whole genome data of eucalyptus,the characteristics of nucleic acid sequence,gene structure,physicochemical properties of protein,conserved domain of protein,phylogenetic relation of family and expression quantity of FLA gene family were analyzed and predicted by Expasy,MEME,MEGA7.0,TBtools and other bioinformatics software.【Result】21 EgrFLAs genes including 3 newly identified EgrFLA genes were presented in eucalyptus,which unevenly distributed in 9 of 11 chromosomes.All identified FLAs contained fasciclin domain,which were clustered into 4 subgroups,with group D including the most gene members.A large number of cis-elements including development elements,salicylic acid,methyl jasmonate related elements were identified in promoters of EgrFLAs genes.Expression profiles in various tissues,adventitious root formation and different stress treatments were also performed.The expression profiles of different tissues showed that except for EgrFLA1,EgrFLA2 and EgrFLA3,EgrFLA5 and EgrFLA7 clustered into group A were also identified to be involved in secondary growth.The expression of EgrFLA1 and EgrFLA3 gradually increased during adventitious root formation in vitro of eucalyptus.The EgrFLA1 and EgrFLA3 were mainly involved in adventitious rootinducing process.The expression of EgrFLA1 and EgrFLA2 significantly altered after SA and MeJA treatments,which meant EgrFLA1 and EgrFLA2 were potentially versatile roles in development and response to stress in eucalyptus.【Conclusion】21 EgrFLAs genes including 3 newly identified EgrFLA genes were presented in Eucalyptus.A large number of cis-elements including development element,phytohormone related elements were identified in promoters of FLAs genes.EgrFLA1,EgrFLA2,EgrFLA3,EgrFLA5 and EgrFLA7 were used as candidate genes in secondary growth and development of eucalyptus,which will provide valuable resources for further molecular breeding in eucalyptus.
作者
于茹恩
曾炳山
范春节
YU Ruen;ZENG Bingshan;FAN Chunjie(College of Biological Science and Technology,Beijing Forestry University,Beijing 100083,China;Key Laboratory of State Forestry Administration on Tropical Forestry,Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou 510520,Guangdong,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2024年第9期127-137,共11页
Journal of Central South University of Forestry & Technology
基金
中国林业科学研究院基本科研业务费专项资金项目(CAFYBB2020ZB004)
“十四五”重点研发计划项目(2021YFD2200102-05)。
关键词
巨桉
类成束状阿拉伯半乳糖蛋白
次生生长
基因家族
基因表达
发育
Eucalyptus grandis
fasciclin-like arabinogalactan proteins
secondary growth
gene family
gene expression
development
