摘要
目的 探究结缔组织病(connective tissue disease,CTD)合并肺间质病变(interstitial lung disease,ILD)患者血清中miR-133a和miR-424表达及与T淋巴细胞亚群的关系。方法 选取我院2019年12月至2022年12月收治的96例CTD-ILD患者为CTD-ILD组,选择同期我院收治的CTD无ILD患者96例为对照组。采用实时荧光定量PCR(qRT-PCR)法检测血清miR-133a和miR-424水平;通过流式细胞仪检测T淋巴细胞亚群水平。采用Pearson法分析miR-133a、miR-424与T淋巴细胞亚群的关系。结果 与对照组相比,CTD-ILD组血清中miR-133a水平显著降低,miR-424的表达水平显著升高(P<0.05)。不同肺通气障碍程度下,轻度、中度和重度患者血清中miR-133a表达水平依次显著降低,miR-424表达水平依次显著升高(P<0.05)。不同肺弥散功能障碍分级下,轻度、中度和重度患者血清中miR-133a表达水平依次显著降低,miR-424表达水平依次显著升高(P<0.05)。CTD-ILD组CD4^(+)、CD4^(+)/CD8^(+)水平与对照组相比显著升高,CD8^(+)、CD3^(+)水平显著降低(P<0.05)。miR-133a与CD4^(+)均呈负相关关系,与CD8^(+)、CD3^(+)呈正相关关系;miR-424与CD4^(+)均呈正相关关系,与CD8^(+)、CD3^(+)呈负相关关系(P<0.05)。结论 CTD-ILD患者血清miR-133a表达水平降低,miR-424表达水平升高,且与患者T淋巴细胞亚群有关。
To investigate the expression of miR-133a and miR-424 in the serum of patients with connective tissue disease(CTD)and interstitial lung disease(ILD)and their relationship with T lymphocyte subpopulations,total of 96 CTD-ILD patients treated in our hospital from December 2019 to December 2022 were selected as CTD-ILD group,while 96 CTD patients without ILD were as the control group.The real-time fluorescence quantitative PCR(qRT-PCR)method was applied to detect serum levels of miR-133a and miR-424;flow cytometry was applied to detect the levels of T lymphocyte subpopulations.Pearson method was applied to analyze the relationship of miR-133a and miR-424 with T lymphocyte subpopulations.Compared with the control group,the level of serum miR-133a in the CTD-ILD group was obviously reduced,while the expression level of miR-424 was obviously increased(P<0.05).Under different degrees of pulmonary ventilation disorders,the expression level of miR-133a in the serum of mild,moderate,and severe patients decreased obviously,while the expression level of miR-424 increased obviously(P<0.05).Under different grades of pulmonary diffusion dysfunction,the expression level of miR-133a in the serum of mild,moderate,and severe patients reduced obviously,while the expression level of miR-424 increased obviously(P<0.05).Furthermore,the levels of CD4^(+)and CD4^(+)/CD8+in the CTD-ILD group were obviously increased,as compared to the control group,while the levels of CD8+and CD3+were obviously reduced(P<0.05).In addition,miR-133a was negatively correlated with CD4^(+),and positively correlated with CD8+and CD3+;miR-424 was positively correlated with CD4^(+),and negatively correlated with CD8+and CD3+(P<0.05).In conclusion,the expression level of miR-133a in serum of CTD-ILD patients is decreased,while the expression level of miR-424 is increased,and both of them are related to the T lymphocyte subpopulations.
作者
赵悦
杨金良
罗寰
席文秀
王珺璐
郑学军
ZHAO Yue;YANG Jinliang;LUO Huan;XI Wenxiu;WANG Junlu;ZHENG Xuejun(Department of Rheumatology and Immunology,First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)
出处
《免疫学杂志》
CAS
CSCD
2024年第4期383-387,共5页
Immunological Journal
基金
2023年度河北省医学科学研究课题(20231416)。
