摘要
试验旨在探究苍朴乌梅散对机体免疫功能的调节作用。使用环磷酰胺诱导小鼠免疫抑制,采用不同剂量的苍朴乌梅散对免疫抑制小鼠和正常小鼠进行进灌胃。选取128只昆明小鼠,雌、雄各半,随机分成8组,每组16只,分别为空白对照组、环磷酰胺模型组、苍朴乌梅散低剂量组(0.8 g/kg BW)、苍朴乌梅散中剂量组(1.6 g/kg BW)、苍朴乌梅散高剂量组(3.2 g/kg BW)、环磷酰胺+苍朴乌梅散低剂量组、环磷酰胺+苍朴乌梅散中剂量组、环磷酰胺+苍朴乌梅散高剂量组。试验期7 d。结果显示,各剂量苍朴乌梅散连续给药7 d能够明显升高免疫抑制小鼠血清中白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、免疫球蛋白G(IgG)和白细胞介素-1β(IL-1β)的水平,提高小鼠体重、脾脏指数和胸腺指数,可以修复环磷酰胺诱导的脾小体萎缩、胸腺结构不清、淋巴细胞变性坏死等病变,且中剂量效果较好。研究表明,苍朴乌梅散能够拮抗环磷酰胺诱导的免疫抑制,修复脾脏和胸腺的损伤,具有增强免疫的作用。综合细胞因子等各项指标及病理组织学结果,推荐苍朴乌梅散的使用剂量为1.6 g/kg BW。
The experiment was designed to investigate the modulating effect of Cangpu Wumei powder on the immune function of the organism.Cyclophosphamide was used to induce immunosuppression in mice,and different doses of Cangpu Wumei powder were administered to both immunosuppressed and normal mice via gavage.A total of 128 Kunming mice,half male and half female,were randomly divided into eight groups,with 16 mice in each group:The blank control group,the cyclophosphamide model group,the low-dose Cangpu Wumei powder group(0.8 g/kg BW),the medium-dose Cangpu Wumei powder group(1.6 g/kg BW),the high-dose Cangpu Wumei powder group(3.2 g/kg BW),the cyclophosphamide+low-dose Cangpu Wumei powder group,the cyclophosphamide+medium-dose Cangpu Wumei powder group,and the cyclophosphamide+high-dose Cangpu Wumei powder group.The experimental period lasted for seven days.The results showed that the administration of each dose of Cangpu Wumei powder for seven consecutive days could significantly increase the levels of interleukin-2(IL-2),interleukin-6(IL-6),immunoglobulin G(IgG),and interleukin-1β(IL-1β)in the serum of immunosuppressed mice,increase the body weight,spleen index,and thymus index of mice,and repair the atrophy of splenic follicles,unclear thymic structure,and necrosis of lymphocytes induced by cyclophosphamide,with the medium dose showing the best effect.The experiment shows that Cangpu Wumei powder can antagonise the immunosuppression induced by cyclophosphamide,repair the damage of spleen and thymus,and has an immune-enhancing effect.Based on the results of cytokines and histopathologic findings,the best dosage of Cangpu Wumei powder to enhance the immunological function in mice is 1.6 g/kg BW.
作者
方人来
赵薛
涂玉佩
易首利
关铭
胡庭俊
FANG Ren-lai;ZHAO Xue;TU Yu-pei;YI Shou-li;GUAN Ming;HU Ting-jun
出处
《饲料研究》
CAS
北大核心
2024年第18期63-69,共7页
Feed Research
基金
南宁市科学研究与技术开发计划项目(项目编号:20232039)
广西研究生教育创新计划项目(项目编号:JGY2023015)
广西大学-大学生创新创业训练计划(项目编号:20220100619、S202310593294)
广西大学巴马产教融合研究院人才科技专项(项目编号:20220024)。
关键词
苍朴乌梅散
小鼠
免疫增强作用
Cangpu Wumei powder
mice
immunostimulatory effect
