摘要
生物固碳是一种经济环保且环境友好的CO_(2)固定技术。该研究基于第二代高通量测序及实时荧光定量PCR技术,探明含油固废残渣中微生物群落结构及主要固碳途径,并采用无碳源富集培养及稳定碳同位素示踪方法,构建含油固废残渣中高效固碳微生物菌群并验证其固碳潜力。16S rDNA结果表明,含油固废残渣中微生物群落多样性和丰富度呈DQ1>DQ2≈LH1>WY>XJ>LH2的趋势,其中,排名前三的门水平物种为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)及厚壁菌门(Firmicutes);荧光定量PCR结果显示,典型固碳功能基因cbbM(1.30×10^(9) copies/g)和cbbL-R(5.28×10^(8) copies/g)丰度最高,比fhs、aclB、accA等固碳基因高2个数量级以上;固碳菌筛选结果显示,产碱杆菌(Alcaligenes faecalis)、人苍白杆菌(Brucella anthropic)、施氏假单胞菌(Stutzerimonas frequens)的RubisCO酶酶活性最高,依次为(36.36±0.81)、(43.83±0.84)、(35.84±1.20)U/g;选用上述3种固碳菌构建高效复合菌群并利用稳定碳同位素示踪方法测算其碳同化量及碳同化速率,结果显示,与未施加菌液的含油固废残渣相比,施加复合菌群S7的含油固废残渣样品微生物碳同化量和碳同化速率分别为(1.30±0.22)mg/kg、(0.14±0.02)mg C/(m^(2)·d),均提升78.08%±7.36%。
Biological carbon sequestration is a hotspot CO_(2) fixation technology due to its ecological,cost-effective and envi⁃ronmental characteristics.Based on the the next generation high throughput sequencing and real-time fluorescence quantita⁃tive PCR technology,this study explored the microbial community structure and main carbon fixation pathways in oily solid waste residues.Using carbon-free enrichment culture microbial technology and stable carbon isotope tracing technology is to screen and construct high-efficiency carbon-fixing microbial flora in oily solid waste residue and prove its carbon-fixing potential.The results of 16S rDNA showed that the microbial community diversity and abundance of oily solid waste resi⁃dues showed a trend of DQ1>DQ2≈LH1>WY>XJ>LH2.The top three phylum-level species of oily solid waste residue were Proteobacteria,Actinobacteria and Firmicutes.The results of real-time fluorescence quantitative PCR showed that the abundance of typical carbon fixation functional genes cbbM(1.30×10^(9) copies/g sample)and cbbL-R(5.28×10^(8) copies/g sample)was the highest,which was more than two orders of magnitude higher than fhs,aclB and accA.Screening results of carbon-fixing bacteria showed that,the RubisCO enzyme activities of Alcaligenes faecalis,Brucella anthropic and Stutzerimonas frequens were the highest,which were(36.36±0.81)U/g,(43.83±0.84)U/g and(35.84±1.20)U/g,respective⁃ly.The above three kinds of carbon-fixing bacteria were used to construct a high-efficiency composite flora and the stable carbon isotope tracing technique was used to measure the carbon assimilation amount and carbon assimilation rate.The results showed that the carbon assimilation amount and carbon assimilation rate of S7 bacteria solution were(1.30±0.22)mg/kg and(0.14±0.02)mgC/(m^(2)·d),respectively,compared with the oily solid waste residue without S7 bacteria solution,the carbon assimilation amount and carbon assimilation rate were increased by 78.08%±7.36%.
作者
宋佳宇
彭梓怡
李兴春
付爱民
任金蔓
SONG Jiayu;PENG Ziyi;LI Xingchun;FU Aimin;REN Jinman(State Key Laboratory of Petroleum Pollution Control,Beijing 102206,China;CNPC Research Institute of Safety and Environment Technology,Beijing 102206,China;College of Chemical Engineering and Environment,China University of Petroleum-Beijing,Beijing 102249,China;Daqing Oilfield Water Environmental Protection Company,Daqing 163712,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2024年第10期84-96,共13页
Environmental Science & Technology
基金
中国石油天然气股份有限公司科技项目(2023ZZ1303,2021DQ03-A4)。
