摘要
目的研究2022年10月至2023年6月安徽省蚌埠市儿童急性呼吸道感染中人腺病毒(HAdV)感染情况和基因进化分析,为呼吸道病毒感染性疾病的防控提供基础数据和科学依据。方法以2022年10月至2023年6月蚌埠市某三甲医院收集的589份急性儿童呼吸道感染住院的儿童病例为研究对象,使用实时荧光定量聚合酶链式反应(qPCR)方法对15种呼吸道病毒进行检测。使用Hep-2细胞对HAdV阳性样本进行病毒分离。使用普通PCR方法扩增HAdV的Hexon基因Loop2高变区域,初步鉴定HAdV分离株的型别;再扩增Hexon基因和Fiber基因,进行基因组进化特征分析。结果589份急性呼吸道感染病例咽拭子样本中共检出病毒阳性184份,检出率为31.24%。HAdV核酸阳性80份(13.58%),其中单一感染63份(78.75%),双重感染16份(20.00%),三重感染1份(1.25%)。80例HAdV阳性病例中,男女性别差异无统计学意义;在不同年龄组中,检出率最高的年龄组为6月龄至2岁组(21.67%)。HAdV阳性患者中临床诊断为肺炎的占56.25%(重症肺炎占22.50%),其次为上呼吸道感染(30.00%)。分离的30株HAd V毒株以HAd V-C1型为主,共24株,HAd V-C2型3株,HAd V-C57型2株,HAd V-C5型1株。结论HAdV是引起2022年10月至2023年6月蚌埠市儿童呼吸道感染的重要病原体,6月龄至2岁婴幼儿为主要感染人群,临床诊断以肺炎为主,应加强对HAdV的监测和防治。
Objective To analyze the infection status and genetic evolution of human adenovirus(HAdV)in children with acute respiratory tract infections in Bengbu,Anhui province,from October 2022 to June 2023,and provide basic data and scientific evidence for the prevention and control of respiratory tract viral diseases.Methods A total of 589 throat swab were collected from hospitalized children with acute respiratory infection in a gradeⅢhospital in Bengbu from October 2022 to June 2023 for the detections of 15 common respiratory viruses by quantitative real-time polymerase chain reaction(qPCR),and Hep-2 cells was used for HAdV isolation in HAdV positive samples.The Loop2 hypervariable region of HAdV Hexon gene was amplified by PCR to identify the type of HAdV isolates.Hexon gene and Fiber gene were amplified to analyze the characteristics of genome evolution of HAdV.Results A total of 184 virus-positive samples were detected in 589 throat swabs from acute respiratory infection cases,and the detection rate was 31.24%.HAdV nucleic acid was detected in in 80 positive samples(13.58%),including 63 samples which were only positive for HAdV(78.75%),16 samples which were positive for HAdV and other 1 virus(20.00%)and 1 sample which was positive for HAdV and other 2 viruses(1.25%).There was no significant difference in HAdV positive rate between boys and girls.In the different age groups,the highest detection rate was in age group 6 months-2 years(21.67%).The main clinical diagnosis was pneumonia(56.25%),and severe pneumonia accounted for 22.50%,followed by upper respiratory tract infection(30.00%).In 30 HAdV isolates,24 were HAdV-C1,3 were HAdV-C2,2 were HAdV-C57 and 1 was HAdV-C5.Conclusion HAdV is an important pathogen of acute respiratory tract infection in children in Bengbu from October 2022 to June 2023,and the infection cases were mainly infants or young children aged 6 months-2 years.Pneumonia was mainly diagnosed.The surveillance and prevention of HAdV infection should be strengthened.
作者
王璐璐
宗可鑫
程迁
陈鑫玥
段婷
宫悦
宋芹芹
宋娟
韩俊
杜海军
陆国玉
Wang Lulu;Zong Kexin;Cheng Qian;Chen Xinyue;Duan Ting;Gong Yue;Song Qinqin;Song Juan;Han Jun;Du Haijun;Lu Guoyu(The First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2024年第9期1127-1134,共8页
Disease Surveillance
基金
国家重点研发计划(No.2022YFC2602402)
国家病原微生物资源库(No.NPRC-32)
传染病预防控制国家重点实验室发展基金(No.2011SKLID104)。
关键词
急性呼吸道感染
人腺病毒
病毒分离
基因特征
Acute respiratory tract infection
Human adenovirus
Virus isolation
Genetic characteristic
