摘要
为满足对动物模型血管结构与氧合功能进行高分辨率、大视场的成像监测需求,提出一种基于光纤超声传感器的光声显微成像技术。该技术采用振镜与电机组合扫描的方式,实现了对动物模型血管结构的高分辨率成像。通过利用不同血氧饱和度下血红蛋白吸收谱的差异,采用双波长激发方式获取血氧饱和度信息。应用该技术对麻醉状态下小鼠脑皮层进行了大范围光声功能成像,观察到麻醉剂对活体血流动力学的差异性影响。此外,对过敏性休克大鼠肠道表面的微循环状态变化进行了成像研究,观察到了过敏性休克状态下血液循环功能的异常变化。实验结果表明,该系统能够在厘米量级范围内,以单血管级空间分辨率对活体动物微循环功能异常进行可视化。该技术为研究疾病过程中血液循环状态的变化提供了新的技术手段。
The condition of blood circulation plays an important role in the development of the disease.In order to monitor the tissue microcirculation of the brain,gastrointestinal tract and other important organs in animal models with a large field of view,and obtain the blood vessel structure and oxygenation function information of target tissues with high sensitivity,high resolution and high contrast,a photoacoustic microscopic imaging technology based on optical fiber ultrasonic sensor was proposed in this paper.In this technology,the orthogonal dual-frequency fiber laser independently developed by our research group is used as the ultrasonic sensor.Different from the traditional piezoelectric ultrasonic sensor,the fiber laser ultrasonic sensor is small in size and carries out ultrasonic sensing in a non-focusing way,which can ensure the detection sensitivity while realizing the large field of view ultrasonic detection.The combined scanning mode of galvanometer and motor is designed,which can realize the large-field imaging of the vascular structure of animal model.Using 532 nm pulsed laser with high power and high repetition frequency as seed light source,on the basis of 558 nm pulsed laser obtained by optical fiber stimulated Raman scattering effect,the blood oxygen saturation information of blood vessels in animal tissues was obtained by dualwavelength excitation method of 532 nm and 558 nm by utilizing the difference of hemoglobin absorption spectrum under different blood oxygen saturation.The imaging results were able to distinguish arteries and veins by different blood vessel colors.By vertically scanning the edge of the surgical blade,the resolution of the large-field optical fiber photoacoustic microimaging system was determined to be 4.2μm.A wide range of photoacoustic functional imaging was performed on the cerebral cortex of mouselet under anesthesia,and the differential effects of different anesthetics on the hemodynamics of live animals were observed,that is,the venous oxygen saturation of live animals under isoflurane and oxygen mixed gas anesthesia was higher,while the arterial blood oxygen saturation was slightly lower than that of pentobarbtal sodium injection.In addition,the changes of microcirculation status on the intestinal surface of rats with anaphylactic shock were studied by wide-field imaging.Abnormal changes of blood circulation function were observed during anaphylactic shock,that is,the mean blood oxygen saturation of venous vessels on the surface of small intestine of rats with shock was lower than that in normal state,and the blood vessels were dilated.The mean blood oxygen saturation of arterial vessels increased,the blood vessels dilated,and the total blood vessels decreased.These results provide effective information for the study of the effect of anaphylactic shock on microcirculation.Experimental results of scanning imaging in animal models show that the system can visualize changes in microcirculatory dysfunction in living animal tissues with single-vessel spatial resolution in the range of centimeters,while obtaining hemodynamic information of blood vessel structure(including blood vessel diameter,blood vessel density),hemoglobin concentration and blood oxygen saturation.This technique provides a new technical means for clinical study of the changes of blood circulation during the occurrence,development and treatment of diseases.
作者
程欢
仲晓轩
曾生
许毓楷
梁贻智
白雪
麦聪
金龙
CHENG Huan;ZHONG Xiaoxuan;ZENG Sheng;XU Yukai;LIANG Yizhi;BAI Xue;MAI Cong;JIN Long(Guangdong Key Laboratory of Optical Fiber Sensing and Communication Technology,College of Physics and Optoelectronics Engineering,Jinan University,Guangzhou 510632,China;Guangdong Institute of Cardiovascular Diseases,Guangdong Academy of Medical Sciences,Guangdong Provincial People's Hospital,Guangzhou 510080,China)
出处
《光子学报》
EI
CAS
CSCD
北大核心
2024年第10期3-10,共8页
Acta Photonica Sinica
基金
国家自然科学基金(Nos.62122031,62275104,62205125,62322506)
广东省基础与应用基础研究基金(No.2023A1515220038,2022A1515010908)
广州市重点领域研发计划(Nos.2024B03J1288,2024B03J0254)。
关键词
光声显微
光纤超声传感器
血流动力学
功能成像
Photoacoustic microscopy
Optical fiber ultrasonic sensor
Hemodynamics
Functional imaging
