摘要
为了探究海水入侵背景下沉积物剖面内微生物群落结构及多样性的垂向变化规律,采集山东省潍坊市莱州湾南岸一眼钻孔深度为200 m的沉积物样品,基于高通量测序方法分析沉积物微生物的16S rRNA基因。结果表明:沉积物全盐量随着深度的增加而逐渐减小,酸碱度随深度的增加而逐渐增大,微生物丰度和α多样性随着深度的增加而降低,变形菌Proteobacteria广泛存在于沉积物中,厚壁菌Firmicutes和拟杆菌Bacteroidete随深度的增加而增加,放线菌Actinobacteria、绿湾菌Chloroflexi、酸杆菌Acidobacteria等随深度的增加而减少;沉积物微生物群落结构的变化特征受多种环境要素共同影响,对微生物群落结构影响最大的是深度和酸碱度,其次是全盐量和总氮。
To explore the vertical changes of microbial community structure and diversity in sediment profiles under the background of transgression,16S rRNA gene analysis of sediment microbe was conducted based on high-throughput sequencing method in sediments from a 200 m deep well in the South Bank of Laizhou Bay in Weifang City,Shandong Province.The results show that the total salt content of sediments gradually decreases with increasing depth,and pH increases with depth.The microbial abundance andαdiversity decrease with the increase of depth.Proteobacteria exists widely in the sediment,Firmicutes and Bacteroidete increase with the increase of depth.Actinobacteria,Chloroflexi,and Acidobacteria decrease with the increase of depth.The characteristics of microbial community structure in sediments are affected by various environmental factors,and depth and pH are the most important factors,followed by total salt content and total nitrogen.
作者
黄宪瑞
支传顺
胡晓农
陈宏帆
陈耿
HUANG Xianrui;ZHI Chuanshun;HU Xiaonong;CHEN Hongfan;CHEN Geng(School of Water Conservancy and Environment,University of Jinan,Jinan 250022,Shandong,China)
出处
《济南大学学报（自然科学版）》
CAS
北大核心
2024年第6期655-663,共9页
Journal of University of Jinan(Science and Technology)
基金
国家自然科学基金项目(42202277)
山东省自然科学基金项目(ZR2022QD024)
山东省高等学校青创科技支持计划项目(2023KJ318)
济南市“新高校20条”引进创新团队项目(2021GXRC070)
济南大学科技计划项目(XBS2202)。
关键词
海(咸)水入侵
水文地质
沉积物
高通量测序
微生物群落
sea(salty)water intrusion
hydrogeology
sediment
high-throughput sequencing
microbial community
