摘要
Δ12/Δ15脂肪酸脱饱和酶(FADS12/15)广泛存在于植物、动物和微生物中,是多不饱和脂肪酸(polyunsaturated fatty acids,PUFAs)合成的关键酶,在其生产中发挥着重要作用。PUFAs具有广泛的益生功能,但是由于人体内缺乏FADS12/15,无法合成亚油酸和α-亚麻酸,因此必须通过食物摄入来补充。此外,人体对其他PUFAs的需求也很高,迫切需要开发优质的PUFAs生产者。微生物是脂肪酸生产的潜在资源,通过FADS12/15基因工程改造可有效提高ω-3和ω-6 PUFAs的产量,从而为人类提供优质的生物脂质资源。作者综述了FADS12/15参与的脂肪酸合成途径及其催化机理,以及FADS12/15基因过表达体系和基因工程微生物种类,总结了FADS12/15同源或异源表达生产PUFAs的研究现状,旨在为FADS12/15基因工程研究和PUFAs的高效生产提供参考。
Fatty acid desaturase,namelyΔ12/Δ15(FADS12/15)are widespread in plants,animals,and microorganisms.They are involved in the biosynthesis of polyunsaturated fatty acids(PUFAs)and play an important role in the production of PUFAs.PUFAs provide various biological and physiological benefits,however,due to the lack of FADS12/15 in the human body,linoleic acid andα-linolenic acid cannot be synthesized.Therefore,their intake must be derived from the diet.Furthermore,other PUFAs are essential for improving diet and human health.Thus,it is urgent to develop high-level PUFA producers.Microorganisms are potential fatty acid producers,and FADS12/15 genetic engineering has been performed to increase the yield ofω-3/ω-6 PUFAs,which provide humans with high-quality lipid resources.This article summarizes the fatty acid biosynthetic pathways and the catalytic mechanism of FADS12/15.It also reviews FADS12/15 overexpression system and genetically engineered microorganisms for PUFA production as well as the homologous or heterologous expression of FADS12/15 gene to produce PUFAs.The objective of this review is to provide the fundamental theory and efficient expression strategy for FADS12/15 genetic engineering to produce PUFAs.
作者
吴琛
史海粟
陈海琴
WU Chen;SHI Haisu;CHEN Haiqin(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;College of Food Science,Shenyang Agricultural University,Shenyang 110866,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2024年第7期1-11,34,共12页
Journal of Food Science and Biotechnology
基金
国家自然科学基金优秀青年基金项目(31722041)。
关键词
Δ12/Δ15脂肪酸脱饱和酶
多不饱和脂肪酸
基因工程微生物
异源表达
催化机制
Δ12/Δ15 fatty acid desaturase(FADS12/15)
polyunsaturated fatty acids
genetically engineered microorganisms
heterologous expression
catalytic mechanism
