摘要
目的探讨酒精性心肌病(ACM)细胞模型中凝集素样氧化低密度脂蛋白受体-1(LOX-1)对细胞凋亡有关蛋白Bcl-2相关蛋白(Bax)、含半胱氨酸的天冬氨酸水解酶3(Cleaved Caspase-3)表达量变化的影响。方法选用若干取对数生长期的细胞,随机分为以下6组:正常对照组(Blank组)、酒精性心肌病组(ACM组)、ACM+sh-NC抑表达对照组(ACM+sh-NC组)、ACM+sh-LOX-1组、ACM+OE-LOX-1过表达组(ACM+OE-LOX-1组)、ACM+OE-LOX-1+si-NC组。除Blank组外,其余各组细胞均用200 mmol/L酒精孵育1 d,体外诱发ACM细胞模型。应用Western blot、实时定量PCR技术分别在蛋白质及基因水平检测细胞中的LOX-1、Bax、Cleaved Caspase-3的表达量并采用Pearson积矩相关系数检验分析其相关性。结果与Blank组相比,ACM组心肌组织中LOX-1、Bax、Cleaved Caspase-3的mRNA表达量显著增加(P<0.01),蛋白表达量同样显著增加(P<0.05);对LOX-1表达进行干预处理后与ACM组及ACM+sh-NC组比较,ACM+sh-LOX-1组心肌组织中Bax、Cleaved Caspase-3的表达量显著减少(P<0.01);与ACM组相比较,ACM+OE-LOX-1组心肌组织中Bax、Cleaved Caspase-3的表达量显著升高(P<0.01);相关性分析提示LOX-1的表达量与Bax、Cleaved Caspase-3的表达量呈正相关(r=0.663,P<0.05;r=0.604,P<0.05)。结论LOX-1在ACM细胞模型中的表达量升高,且与心肌细胞凋亡蛋白Bax、Cleaved Caspase-3表达呈正相关,并通过干预Bax、Cleaved Caspase-3表达参与酒精诱导心肌细胞凋亡的过程。
Objective To investigate the effect of lectin-like oxidized low-density lipoprotein receptor-1(LOX-1)on the expression of apoptosis-related proteins in an alcohol cardiomyopathy(ACM)cell model.Methods A number of cells of logarithmic growth phase were selected and randomly divided into the following six groups:normal control group(Blank group),alcoholic cardiomyopathy group(ACM group),ACM+sh-NC inhibition control group(ACM+sh-NC group),ACM+sh-LOX-1 group,ACM+OE-LOX-1 overexpression group(ACM+OE-LOX-1 group),ACM+OE-LOX-1+si-NC group.Except for the blank group,all groups of cells were incubated with 200 mmol/L alcohol for 1 d to induce alcoholic cardiomyopathy cell model in vitro.The expression levels of LOX-1,Bax,Cleaved Caspase-3 were detected at the protein and gene levels by Western blot and real-time quantitative PCR,respectively,and the correlation was analyzed by Pearson product moment correlation coefficient.Results The expression of LOX-1,Bax and Cleaved Caspase-3 in myocardial tissue was significantly increased in the ACM group compared with the Blank group(P<0.01);the protein expression was also significantly increased(P<0.05).After intervening with LOX-1 expression,the expression of Bax and Cleaved Caspase-3 in myocardial tissue was significantly decreased in the ACM+sh-LOX-1 group compared with the ACM and ACM+sh-NC groups(P<0.01).The expression of Bax and Cleaved Caspase-3 in myocardial tissues was significantly increased in the ACM+OE-LOX-1 group compared with the ACM group(P<0.01);correlation analysis suggested that the expression of LOX-1 was positively correlated with the expression of Bax and Cleaved Caspase-3(r<0.01).Expression was positively correlated with the expression of Bax and Cleaved Caspase-3(r=0.663,P<0.05;r=0.604,P<0.05).Conclusion The expression of LOX-1 is increased in the alcoholic cardiomyopathy cell model,and is positively correlated with the expression of cardiomyocyte apoptosis proteins Bax and CleavedCaspase-3,and participates in the alcohol-induced apoptosis of cardiomyocytes by interfering with the expression of Bax and CleavedCaspase-3.
作者
郭明燕
刘东
周娜
王鑫
袁博
冯占斌
张一凡
GUO Mingyan;LIU Dong;ZHOU Na;WANG Xin;YUAN Bo;FENG Zhanbin;ZHANG Yifan(Graduate School of Medicine,Xi′an Medical University,Xi′an 710021,China;Cardiovascular Hospital,Xi′an Ninth Hospital,Xi′an 710054,China)
出处
《西部医学》
2024年第11期1582-1587,共6页
Medical Journal of West China
基金
西安市创新能力强基计划-医学研究项目(21YXYJ0066)。
