摘要
目的:对炆何首乌治疗雄激素性脱发(AGA)的作用效果进行评价,并对其作用机制进行研究。方法:99只SPF级雄性C57BL/6J小鼠按照随机数字表法分为空白组、模型组、阳性药非那雄胺组(0.65 mg·kg^(-1))、炆/制何首乌低、中、高剂量组(0.78、1.56、3.12 g·kg^(-1))。利用丙酸睾酮稀释液皮下多点注射60 d构建小鼠AGA模型,并于第11天开始灌胃给药。通过脱毛区域的新生毛发面积、毛发长度、毛发质量、苏木素-伊红(HE)染色评价炆/制何首乌对AGA的治疗作用;进一步利用酶联免疫吸附测定法(ELISA)检测小鼠皮肤组织中睾酮(T)、双氢睾酮(DHT)和5α-还原酶(5-AR)的含量,利用蛋白免疫印迹法(Western blot)对Wnt/β-连环蛋白(β-catenin)信号通路中的关键蛋白进行验证。结果:造模60 d后,与空白组比较,模型组小鼠背部脱发区域的新生毛发面积、毛发长度及质量、含黑色素毛囊数量/总毛囊数量比值明显降低(P<0.05,P<0.01),皮肤组织中T、DHT、5-AR含量及糖原合酶激酶-3β(GSK-3β)表达水平明显升高(P<0.05,P<0.01),β-catenin、磷酸化糖原合酶激酶-3β(p-GSK-3β)与p-GSK-3β/GSK-3β表达水平明显降低(P<0.05,P<0.01);与模型组比较,非那雄胺、炆/制何首乌低、中、高剂量能够显著增加小鼠新生毛发面积及长度(P<0.01),炆何首乌低、中剂量明显升高新生毛发质量(P<0.05,P<0.01),非那雄胺、炆何首乌低、中、高剂量及制何首乌低、高剂量明显升高小鼠皮肤组织中含黑色素毛囊数量/总毛囊数量比值(P<0.05,P<0.01);与相同生药量的制何首乌比较,炆何首乌中、高剂量明显升高含黑色素毛囊数量/总毛囊数量比值(P<0.05)。与模型组比较,炆何首乌能显著降低小鼠皮肤组织中T、DHT含量和GSK-3β表达水平(P<0.01),显著升高β-catenin、p-GSK-3β及p-GSK-3β/GSK-3β表达水平(P<0.05,P<0.01)。结论:炆何首乌能够改善小鼠雄激素性脱发,其作用机制与降低雄性激素水平、促进Wnt/β-catenin信号传导有关。
Objective:To evaluate the therapeutic effect of stewed Polygoni Multiflori Radix on androgenic alopecia(AGA)and study the treatment mechanism.Method:Ninety-nine SPF-grade male C57BL/6J mice were randomized into control,model,positive drug(finasteride,0.65 mg·kg^(-1)),low(0.78 g·kg^(-1)),medium(1.56 g·kg^(-1)),and high(3.12 g·kg^(-1))-dose stewed Polygoni Multiflori Radix,and Polygoni Multiflori Radix Praeparata groups by the random number table method.The mouse model of AGA was constructed by subcutaneous multi-point injection of testosterone propionate diluent for 60 days,and the mice were administrated with corresponding drugs by gavage from day 11.The therapeutic effects of stewed Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata on AGA were evaluated by newly hair area,hair length,hair weight in the hair removal area,and hematoxylin-eosin staining.Enzyme-linked immunosorbent assay was employed to determine the levels of testosterone(T),dihydrotestosterone(DHT),and 5α-reductase(5-AR)in the skin tissue of mice.Western blot was employed to determine the expression levels of key proteins in the Wnt/β-catenin signaling pathway.Result:Compared with the control group,the model group(after 60 days of modeling)showed reductions in the newly hair area,hair length and weight in the back hair removal area,and ratio of hair follicles containing melanin to total hair follicles(P<0.05,P<0.01),elevated levels of T,DHT,and 5-AR,up-regulated expression level of glycogen synthase kinase-3β(GSK-3β)(P<0.05,P<0.01),and down-regulated expression levels ofβ-catenin,phospho-glycogen synthase kinase-3β(p-GSK-3β),and p-GSK-3β/GSK-3β(P<0.05,P<0.01)in the skin tissue.Compared with the model group,the positive drug,low-,medium-,and high-dose stewed Polygoni Multiflori Radix,and low-,medium-,and high-dose Polygoni Multiflori Radix Praeparata improved the newly hair area and hair length of mice(P<0.01),and stewed Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata at low and medium doses improved the weight of newly formed hair in mice(P<0.05,P<0.01).The positive drug,low-,medium-,and high-dose stewed Polygoni Multiflori Radix,and low-and high-dose Polygoni Multiflori Radix Praeparata increased the ratio of hair follicles containing melanin to total hair follicles in the skin tissue(P<0.05,P<0.01).Compared with Polygoni Multiflori Radix Praeparata at the same doses,the medium and high doses of stewed Polygoni Multiflori Radix increased the ratio of melanin-containing hair follicles to total hair follicles(P<0.05).Compared with the model group,stewed Polygoni Multiflori Radix lowered the levels of T and DHT,downregulated the expression level of GSK-3β(P<0.01),and up-regulated the expression levels ofβ-catenin,p-GSK-3β,and p-GSK-3β/GSK-3β(P<0.05,P<0.01)in the skin tissue of the mice.Conclusion:Stewed Polygoni Multiflori Radix can ameliorate androgenic alopecia in mice by reducing the androgen level and promoting Wnt/β-catenin signaling.
作者
潘福竺
陈明霞
易斌
薛艳华
玉秋萍
伍发云
冀恩惠
吴宏伟
许静
PAN Fuzhu;CHEN Mingxia;YI Bin;XUE Yanhua;YU Qiuping;WU Fayun;JI Enhui;WU Hongwei;XU Jing(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Pharmacy,Zunyi Medical University,Zunyi 563099,China;Jianchangbang Pharmaceutical Co.Ltd.,Nancheng 344700,China;Beijing Scrianen Pharmaceutical Co.Ltd.,Beijing 100176,China;Jiangxi Province Key Laboratory of Traditional Chinese Medicine Processing(Stewed Method),Nancheng 344100,China;Civil Aviation General Hospital,Civil Aviation Medical Center of Civil Aviation Administration of China,Beijing 100123,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2024年第23期246-253,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省重点研发计划项目(20223BBG71001)
抚州市“揭榜挂帅”项目(XMBH00031)。
