摘要
目的:利用网络药理学研究蓝盆花总黄酮活性成分的抗非酒精性脂肪性肝炎作用机制。方法:基于TCMSP数据库及文献报道获取蓝盆花总黄酮主要活性成分。依据反向药效团匹配方法采用Swiss Target Prediction服务器预测其活性成分靶标,并通过Genecards、OMIM、Disgenet等数据库筛选出改善非酒精性脂肪性肝炎的靶标。取药物靶点与疾病靶点共同作用的潜在靶点制作韦恩图,使用Cytoscape软件,构建“活性成分-靶点”网络图,利用STRING平台构建蛋白互作网络,利用DAVID数据库与ClueGO插件进行GO富集分析和KEGG通路分析。结果:共筛选得到蓝盆花总黄酮的活性成分43个及靶点439个,网络中主要活性成分为芒柄花苷、莰菲醇-3-O-芸香糖苷、异鼠李素、槲皮素、木犀草素、淫香叶木素等,蛋白互作网络中关键靶点为TNF、AKT1、TP53、VEGFA、IL1B等。GO富集分析共涉及到生物过程503个,细胞组分54个,分子功能120个,KEGG通路富集分析筛选前20条相关通路,主要作用于VEGF信号通路、PPAR信号通路、雌激素信号通路等。结论:蓝盆花的黄酮成分可通过多靶标、多通路发挥治疗非酒精性脂肪性肝炎的作用,该结果为深入研究蓝盆花在抗非酒精性脂肪性肝炎等领域的作用提供了科学依据。
Objective:To study the anti-nonalcoholic steatohepatitis mechanism of total flavonoids from Scabiosa comosa by network pharmacology.Methods:Based on the TCMSP database and literature reports,the main active components of total flavonoids from Scabiosa comosa were obtained.The Swiss Target Prediction server was used to predict the target of its active ingredients according to the reverse pharmacophore matching method,and the targets for improving NASH were screened out through databases such as Genecards,OMIM and Disgenet.The potential targets of drug targets and disease targets were used to make the Venn diagram.The Cytoscape software was used to construct the'active ingredient-target'network diagram.The STRING platform was used to construct the protein interaction network.The DAVID database and ClueGO plug-in were used for GO enrichment analysis and KEGG pathway analysis.Results:A total of 43 active components and 439 targets of total flavonoids were screened.The main active components in the network were ononin,camphene-3-O-rutinoside,isorhamnetin,quercetin,luteolin,eupatorium odoratum,etc.The key targets in the protein interaction network were TNF,AKT1,TP53,VEGFA,IL1B,etc.GO enrichment analysis involved 503 biological processes,54 cellular components and 120 molecular functions.KEGG pathway enrichment analysis screened the top 20 related pathways,mainly acting on VEGF signaling pathway,PPAR signaling pathway,estrogen signaling pathway and so on.Conclusion:The flavonoid components from Scabiosa comosa can play a role in the treatment of nonalcoholic steatohepatitis through multiple targets and multiple pathways.The results provide a scientific basis for further study of the role of Scabiosa comosa in the field of anti-nonalcoholic steatohepatitis.
作者
赵玉莲
张娜
ZHAO Yulian;ZHANG Na(School of Pharmacy,Baotou Medical College,Baotou 014040,China)
出处
《包头医学院学报》
CAS
2024年第11期66-72,共7页
Journal of Baotou Medical College
基金
内蒙古自治区自然科学基金(2023LHMS08044)
内蒙古自治区高等学校青年科技英才支持计划资助(NJYT24080)。
关键词
蓝盆花
总黄酮
非酒精性脂肪性肝炎
网络药理学
Scabiosa comosa
Total flavone
Nonalcoholic steatohepatitis
Network pharmacology
