摘要
[目的]本文旨在研究干旱胁迫对葡萄生理特性以及胁迫相关基因表达的影响。[方法]以‘阳光玫瑰’和‘巨峰’葡萄品种为试材,采用人工模拟干旱胁迫的方法,调查2个品种植株的胁迫症状,测定生理指标,并对2个品种在干旱胁迫处理下的各生理指标进行主成分分析和隶属函数分析,同时对叶片胁迫相关基因的表达进行测定。[结果]‘巨峰’和‘阳光玫瑰’2个品种的耐旱性均表现为胁迫前期植株基部叶片出现萎蔫并伴有叶缘焦枯,后期植株叶片失绿萎蔫至枯黄,‘阳光玫瑰’出现胁迫症状早于‘巨峰’。随着干旱胁迫时间的延长,2个葡萄品种的叶片旱情指数增加,株高和茎粗均呈先升高后降低的趋势,叶绿素含量下降,过氧化物酶、超氧化物歧化酶和抗坏血酸过氧化氢酶活性呈先上升后下降的趋势,而光系统Ⅱ(PSⅡ)中非调节能量耗散的量子产额、丙二醛含量、脯氨酸含量和可溶性蛋白含量呈上升趋势。‘巨峰’和‘阳光玫瑰’在干旱胁迫下的平均隶属函数值为0.47和0.46,‘巨峰’对干旱胁迫耐受性略高于‘阳光玫瑰’。RT-qPCR结果显示干旱胁迫处理显著上调2个葡萄品种叶片中VvPIP1、VvWRKY71、VvPP2C2、VvRD22的基因表达量。[结论]干旱胁迫下,‘阳光玫瑰’和‘巨峰’葡萄对干旱胁迫的响应趋势相同但响应程度存在差异,‘巨峰’的干旱耐受性高于‘阳光玫瑰’。
[Objectives]The paper aimed to study the effects of drought stress on the physiological characteristics of grapes and the expression of stress-related genes.[Methods]The stress symptoms of the plants of‘Shine Muscat’and‘Kyoho’grape varieties,serving as test materials,were investigated through artificial drought stress simulation methods.Physiological indicators were measured,and principal component analysis and membership function analysis were conducted on these indicators for the two varieties under drought stress treatment.Furthermore,the expression levels of stress-related genes in the leaves were determined.[Results]The drought tolerance symptoms of both‘Kyoho’and‘Shine Muscat’were characterized by leaf wilting with leaf margin scorching at the base of the plant in the early period of drought stress,and green wilting and yellowing at the end of the stress period,but‘Shine Muscat’showed drought stress symptoms earlier than those of‘Kyoho’.With the prolongation of drought stress time,the leaf drought index of two grape varieties increased,the plant height and stem thickness showed a trend of increasing and then decreasing,the chlorophyll content declined,the activities of the peroxidase,superoxide dismutase,and ascorbate peroxidase showed a trend of increasing and then decreasing,and the quantum yield of the non-regulated energy dissipation in the PSⅡ,malondialdehyde content,proline content and soluble protein content showed an increasing trend.The average affiliation function values of‘Kyoho’and‘Shine Muscat’under drought stress were 0.47 and 0.46 respectively,and‘Kyoho’was slightly more tolerant to drought stress than‘Shine Muscat’.The RT-qPCR results showed that drought stress treatment significantly up-regulated the expression levels of the genes of VvPIP1,VvWRKY71,VvPP2C2 and VvRD22 in the leaves of two grape varieties.[Conclusions]Under drought stress,the response of‘Shine Muscat’and‘Kyoho’grape to drought stress was the same but the degree of response differed,and the drought tolerance of‘Kyoho’was higher than that of‘Shine Muscat’.
作者
徐文清
李少楠
宋思言
陈小蓓
杨思玲
柴文枝
房经贵
XU Wenqing;LI Shaonan;SONG Siyan;CHEN Xiaobei;YANG Siling;CHAI Wenzhi;FANG Jinggui(Jiangsu Agricultural Technology Extension General Station,Nanjing 210036,China;College of Horticulture/Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province,Nanjing Agricultural University,Nanjing 210095,China;Zhenjiang Institute of Agricultural Sciences of Hilly Area of Jiangsu Province,Zhenjiang 212400,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2024年第6期1049-1057,共9页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(32272647)
山东省重点研发计划(农业良种工程)项目(2022LZGCQY1018)
江苏省现代农业产业技术体系建设项目(JATS〔2022〕457)。
关键词
葡萄
干旱胁迫
生理特性
抗氧化
酶活性
grape
drought stress
physiological characteristics
antioxidant
enzyme activity
