摘要
目的探讨黄芪总皂苷对2型糖尿病(T2DM)大鼠肌少症的改善作用。方法将大鼠分为正常组和造模组,造模组大鼠采用高糖高脂饮食结合腹腔注射STZ构建T2DM模型,造模成功的大鼠随机分为模型组、二甲双胍组(0.2 g/kg)和黄芪总皂苷组(80 mg/kg),灌胃给予相应剂量药物。给药12周后测定大鼠FBG、餐后2小时血糖(PG2h)和骨骼肌湿重;ELISA法检测血清INS、C肽(C-P)、IGF-1、TNF-α和IL-1β水平;HE染色观察骨骼肌形态变化;Western blot法检测骨骼肌PI3K、p-Akt、mTOR、S6K1、FoxO1、Murf1蛋白表达;RT-qPCR法检测骨骼肌Pi3k、Akt、mtor mRNA表达。结果与模型组比较,黄芪总皂苷组大鼠FBG、PG2h、OGTT-AUC、HOMA-IR、TNF-α和IL-1β水平降低(P<0.01),INS、C-P、IGF-1水平和骨骼肌湿重升高(P<0.05,P<0.01),骨骼肌萎缩程度改善;骨骼肌PI3K、p-Akt、mTOR、S6K1蛋白表达升高(P<0.05,P<0.01),FoxO1、Murf1蛋白表达降低(P<0.05,P<0.01),Pi3k、Akt、mtor mRNA表达升高(P<0.01)。结论黄芪总皂苷对T2DM大鼠肌少症有改善作用,该作用可能与调控PI3K/Akt/mTOR及PI3K/Akt/FoxO1通路有关。
AIM To investigate the effects of total Astragalus saponins on the improvement of sarcopenia in a rat model of type 2 diabetes mellitus(T2DM).METHODS The rats were divided into the normal group for a normal feeding and the model group for the feeding of high-sugar and high-fat diet combined with intraperitoneal injection of STZ to establish a T2DM model.The successful model rats were randomly divided into the model group,the metformin group(0.2 g/kg)and the total Astragalus saponins group(80 mg/kg),and given corresponding doses of drugs by gavage.After 12 weeks administration,the rats had their FBG,postprandial blood glucose(PG2h)and wet weight of skeletal muscle measured;their serum levels of INS,C-peptide(C-P),IGF-1,TNF-αand IL-1βdetected by ELISA;their morphological changes of skeletal muscle observed by HE staining;their protein expressions of PI3K,p-Akt,mTOR,S6K1,FoxO1 and Murf1 in skeletal muscle detected by Western blot;and their mRNA expressions of Pi3k,Akt and mtor in skeletal muscle detected by RT-qPCR method.RESULTS Compared with the model group,the total Astragalus saponins group displayed decreased levels of FBG,PG2h,OGTT-AUC,HOMA-IR,TNF-αand IL-1β(P<0.01);increased levels of INS,C-P,IGF-1 and wet weight of skeletal muscle(P<0.05,P<0.01);improved skeletal muscle atrophy and increased protein expressions of PI3K,p-Akt,mTOR and S6K1 in skeletal muscle(P<0.05,P<0.01);decreased protein expressions of FoxO1 and Murf1(P<0.05,P<0.01);and increased mRNA expressions of Pi3k,Akt and mtor(P<0.01).CONCLUSION The improvement effects of total Astragalus saponins on sarcopenia in T2DM rats may be associated with the regulation of PI3K/Akt/mTOR and PI3K/Akt/FoxO1 pathways.
作者
马雷雷
李继安
徐文轩
王静雅
田朝阳
李嘉钰
韩茹杰
喇孝瑾
田春雨
常宏
代紫阳
张碧溦
MA Lei-lei;LI Ji-an;XU Wen-xuan;WANG Jing-ya;TIAN Zhao-yang;LI Jia-yu;HAN Ru-jie;LA Xiao-jin;TIAN Chun-yu;CHANG Hong;DAI Zi-yang;ZHANG Bi-wei(School of Public Health,North China University of Science and Technology,Tangshan 063210,China;College of Traditional Chinese Medicine,North China University of Science and Technology,Tangshan 063210,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第11期3612-3619,共8页
Chinese Traditional Patent Medicine
基金
国家科学技术部对发展中国家科技援助项目(KY201904005)
河北省自然科学基金资助项目(H2023209038)
河北省中医药管理局科研计划项目(2020222)
河北省中医药管理局科研计划项目(2022140)。
