摘要
以陇东地区驯化种植的感病黄精为试验材料,采用组织分离法、平板划线法、形态观察法和分子生物学方法分离病原微生物,鉴定了病原菌的种属特性,验证了所分离的病原微生物对黄精健康组织的致病性,并利用9种中药材和5种黄精内生菌探寻对黄精病害的生物防治方法。结果表明:从黄精感病组织中分离得到10株细菌和6株真菌,其中BY-Z1、BY-Z3和BY-Z4这3株真菌具有较强的致病性,经鉴定分别是芳香镰刀菌、腐皮镰刀菌和多孢小孢子菌,其中多孢小孢子菌为首次报道从黄精中分离到的担子菌属病原真菌;黄连对BY-Z1的生长抑制作用最强,抑制率为74.0%,当归次之(28.9%);黄连对BY-Z3的生长抑制作用最强,抑制率为66.0%,丹皮次之(21.6%);黄连对BY-Z4的生长抑制作用最强,抑制率为100.0%,茜草根次之(53.0%);枯草芽孢杆菌对致病真菌BY-Z1、BY-Z3和BY-Z4均具有抑制作用,黄精内生菌H3和H4对真菌BY-Z1和BY-Z4具有抑制作用,H1对BY-Z4有强抑制作用。
This study used locally domesticated and cultivated susceptible Polygonatum sibiricum as the material.Tissue separation,plate streak,morphological observation and molecular biology methods were used to isolate pathogenic microorganisms and identify their species characteristics.The pathogenicity of the isolated pathogenic microorganisms to healthy tissues of Polygonatum sibiricum was verifed,and biological control methods for Polygonatum sibiricum disease were explored by 9 Chinese medicinal materials and 5 endophytic bacteria of Polygonatum.The results show that among the 10 strains of bacteria and 6 strains of fungi isolated from Polygonatum sibiricum infected tissues,3 strains of fungi BY-Z1,BY-Z3 and BY-Z4 have strong pathogenicity.After identification,they are Fusarium redolens,Fusarium solani and Minimedusa polyspora.Minimedusa polyspora is the first reported basidiomycetes isolated from Polygonatum sibiricum.Coptidis rhizoma has the strongest growth inhibition on BY-Z1,with an inhibition rate of 74.0%,followed by Angelica sinensis(28.9%).Coptidis rhizoma has the strongest growth inhibition on BY-Z3,with an inhibition rate of 66.0%,followed by mudan cortex(21.6%).Coptidis rhizoma has the strongest growth inhibition on BY-Z4,with an inhibition rate of 100.0%,followed by roots of madder(53.0%).Bacillus subtilis has inhibitory effects on pathogenic fungi BY-Z1,BY-Z3 and BY-Z4,while endophytic bacteria H3 and H4 of Polygonatum si-biricum have inhibitory effects on BY-Z1 and BY-Z4.H1 has a strong inhibitory effect on BY-Z4.
作者
王博
刘秀丽
白亚静
欧晓彬
孔新年
马婧
刘正锟
WANG Bo;LIU Xiu-li;BAI Ya-jing;OU Xiao-bin;KONG Xin-nian;MA Jing;LIU Zheng-kun(School of Agriculture and Bioengineering,Longdong University,Qingyang 745000,China;Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration,Qingyang 745000,China)
出处
《江西农业学报》
CAS
2024年第11期77-83,共7页
Acta Agriculturae Jiangxi
基金
庆阳市科技计划项目(QY2021A-F002)
国家自然科学基金项目(22107041)
甘肃省高等产业支撑项目(2020C-24)
甘肃省科技计划自然科学基金项目(21JR1RM328)
陇东学院青年科技创新项目(XYZK1901)
陇东学院2024年大学生创新创业训练计划项目(202401092)。
关键词
黄精
病原微生物
分离鉴定
生物防治
Polygonatum sibiricum
Pathogenic microorganism
Isolation and identification
Biological control
