摘要
[目的]为解析小麦(Triticum aestivum L.)白叶突变体叶色白化机制,以白叶突变小麦体和正常小麦为试验材料,从转录水平方向展开研究。[方法]以正常小麦和温敏白叶突变体小麦为材料,进行叶色变化过程的比较转录组学分析,挖掘调控小麦光合色素形成的调控机制。[结果]参与正常小麦叶片发育过程的DGEs数目是白叶小麦叶色由白转绿过程的2.57倍,且正常叶片发育过程的DGEs主要参与脂类、氨基酸、蛋白质、嘌呤、肌醇等生物大分子的代谢,而叶色由白转绿过程的DGEs主要参与光合作用、呼吸作用和糖代谢等过程;同时发现,小麦拥有已知植物光合色素代谢途径中的所有基因,其中的75个基因为DGEs,这些DGEs包含了所有叶绿素合成途径的基因和2个类胡萝卜素合成途径的基因,这些基因的表达模式分为7类(Ⅰ-Ⅶ),表达模式表明小麦叶绿素的生物合成受反馈调节,并且叶绿素合成受阻导致试验材料形成白叶,TaCAO可能是叶绿素合成受阻的主要影响因子。[结论]该研究为进一步研究小麦叶绿素形成机制奠定了基础。
[Objective]To decipher the mechanism of leaf color albinism in Triticum aestivum L.white-leaf mutants.[Method]This study conducted a comparative transcriptomic analysis of the leaf color change process using normal Triticum aestivum L.and temperature-sensitive white-leaf mutants.The aim was to uncover the regulatory mechanisms governing the formation of photosynthetic pigments in Triticum aestivum L..[Result]The results showed that the number of Differentially Expressed Genes(DGEs)involved in the development of normal Triticum aestivum L.leaves was 2.57 times that in the process of white leaf color turning green.DGEs in the normal leaf development process were mainly involved in the metabolism of lipids,amino acids,proteins,purines,and inositol,among other biomacromolecules.In contrast,DGEs in the process of leaf color turning from white to green were primarily involved in photosynthesis,respiration,and carbohydrate metabolism.Furthermore,it was found that Triticum aestivum L.possesses all the genes in the known plant photosynthetic pigment metabolic pathways,75 of which were DGEs.These DGEs included all genes of the chlorophyll synthesis pathway and two carotenoid synthesis pathway genes.Their expression patterns can be classified into seven categories(I to VII).These patterns suggest that the biosynthesis of Triticum aestivum L.chlorophyll is subject to feedback regulation,and that impaired chlorophyll synthesis leads to the formation of white leaves,with TaCAO likely being the main factor affecting chlorophyll synthesis.[Conclusion]These findings provide a theoretical basis for further research into the chlorophyll formation mechanism in Triticum aestivum L..
作者
王志龙
南文智
刘婷婷
何旭光
樊晨晨
李渊博
张雄
WANG Zhi-long;NAN Wen-zhi;LIU Ting-ting(Life Science College of Yulin University,Yulin,Shaanxi 719000)
出处
《安徽农业科学》
CAS
2024年第23期85-93,共9页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(32160462)
榆林学院研究生创新基金项目(2022YLYCX08)。
关键词
小麦
白叶性状
比较转录组学
Triticum aestivum L.
White-leaf trait
Comparative transcriptomics
