摘要
目的 :研究肠安颗粒剂对小鼠及大鼠胃肠运动及胃液分泌功能的影响。方法 :采用清醒状态下灌胃给药 ,测定给药后小鼠小肠内炭末前沿至幽门的距离占小肠全长、大鼠结肠内炭末前沿至回盲瓣的距离占结肠全长的百分比 ,小鼠胃内苯酚红的残留量、大鼠胃液与胃酸的分泌量和胃蛋白酶的活性。苯酚红含量测定采用紫外分光光度法 ,胃蛋白酶的活性采用Mett法。结果 :肠安颗粒剂可增大小鼠小肠内炭末前沿至幽门的距离占小肠全长和大鼠结肠内炭末前沿至回盲瓣的距离占结肠全长的百分比 ,增加大鼠胃液、胃酸的分泌量以及增强胃蛋白酶的活性。可减少用药后 3 0min小鼠胃内苯酚红的残留量。结论 :肠安颗粒剂具有促进胃、小肠和大肠运动和胃液分泌的功能。
Objective:To Study the effect of changan (gut soothing) granules (composed of several traditional Chinese medicinal herbs) on the function of stomach and intestine in the mouse and rat. Method: Changan granules, carbon particles and phenolsulfonphthalein were administered by oral or cecal intubation, respectively to conscious mice and rats. After a definite time period, the ratio of the distance between the leading front of carbon particles in the small intestine and the pylorus to the full length of the small intestine in the mouse, the ratio of the distance between the leading front of carbon particles in the colon and the ileocecal valve to the full length of the colon in the rat, the amount of remnant phenolsulfonphthalein in the stomach of the mouse, the amount of gastric juice and gastric acid secretion as well as the activity of pepsin in the rat, were measured. Phenolsulfonphthalein was determined by ultraviolet spectrophotometry, pepsin activity, with the mett's method. Results:The aforementioned ratios were increased by changan/granules in doses of 0.25, 0.50, 1.00 g·kg 1 in the rat and mouse. The secretion of gastric juice and gastric acid and the activity of pepsin were also increased in the rat. The phenolsulfonphthalein remnant in the stomach of the mouse was decreased 30 min after the administration of changan granules in doses of 0.125, 0.250, 0.500 g·kg 1 . Conclusion: Changan granules were shown to promote the motility of the stomach, small intestine and large intestine as well as the secretory function of the stomach.
出处
《医药导报》
CAS
2002年第12期764-765,共2页
Herald of Medicine
