摘要
目的 利用生物发光法测定人群外周淋巴细胞DNA加合物水平 ,并分析其影响因素 ,为进一步探讨水污染与人群DNA加合物水平之间的关系奠定基础。方法 采用随机整群抽样方法 ,抽取 2 34例健康人群 ,测定其外周淋巴细胞DNA加合物水平 ,并对年龄、吸烟、饮食习惯等进行分析。结果 健康人群中男性DNA加合物水平略高于女性 ,但二者之间差异无显著意义 ;人群DNA加合物与年龄、吸烟、饮茶、饮食习惯和饮酒等因素均有显著相关性 ,其中年龄、吸烟与DNA加合物的相关性最为明显 ;随着年龄的增加 ,DNA加合物水平上升 ;吸烟越多 ,DNA加合物水平越高。结论 人群DNA加合物水平影响因素很多 ,其在人体生物监测、肿瘤形成风险评估中的应用 。
Objective To develop bioluminescence method to measure DNA adducts in human peripheral lymphocytes and study the factors that affect the level of DNA adduct, in order to use it in studying the relationship between water pollution and human DNA adduct levels Method The measurement of DNA adduct in peripheral lymphocytes was carried out in 234 normal persons, and the relationship between DNA adducts levels and age, smoking and eating habit etc was analyzed Results (1) The level of DNA adduct in males was higher than that in females, but without significance ( P >0 05); (2) The level of DNA adduct was significantly correlated with age, smoking, tea drinking, eating habit and alcohol drinking Among them, age plus smoking were the most important factors affected the DNA adduct levels ( P <0 01) The level of DNA adduct increased with age The more cigarette smoked, the higher was the level of DNA adduct Conclusion The level of DNA adduct is affected by many factors, so it needs further research to apply it in the biomonitoring and risk assessment of environment chemicals
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2002年第6期363-365,共3页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金重大项目资助 (3 9990 5 70 )
国家"九五"科技攻关课题 (96 911 0 7 0 2 )
