摘要
目的 探讨汉防己甲素 (Tet)联合屈洛昔芬 (Drol)对耐药细胞系K5 6 2 A0 2的逆转作用及其与诱导凋亡的关系。方法 采用甲基四唑蓝法 (MTT)测定柔红霉素 (DNR)的细胞毒性 ;采用DNA凝胶电泳法观察Tet、Drol单独及联合应用对K5 6 2 A0 2细胞凋亡诱导作用的影响。结果 0 .6 2μg mlTet、1.94 μg mlDrol均能增加DNR对K5 6 2 A0 2的细胞毒作用 ,其半数抑制量IC50 分别为 7.2 8±2 .0 6 μg ml和 7.5 8± 3.4 4 μg ml,逆转倍数分别为 2 .94倍和 2 .82倍。两药联合作用明显增强 ,其IC50为 1.6 6± 0 .4 1μg ml,逆转倍数达 12 .9倍。 0 .6 2 μg mlTet、1.94 μg mlDrol单独及联合应用均不会诱导K5 6 2 A0 2细胞凋亡。结论 单独应用Tet、Drol可部分逆转K5 6 2 A0 2细胞的耐药性 ,两药联用具有明显协同效应。Tet、Drol逆转耐药的机理与诱导K5 6 2 A0 2细胞凋亡无关。
Objective To study the reversal effect of tetrandrine (Tet) and droloxifene (Drol) on multidrug resistant cell line K562/A02 and apoptosis induction. Methods The cytotoxicity of daunorubicin(DNR) was assayed by MTT method. The effects of Tet and DRL, alone or combined were detected through the apoptosis of K562/A02 by agarose gel electrophoresis. Results The cytotoxicity of DNR to K562/A02 was enhanced by 0.62 μg/ml Tet or 1.94 μg/ml Drol with IC_ 50 7.28±2.06 μg/ml, 7.58±3.44 μg/ml, giving a reversal effect of 2.94 and 2.82. But IC_ 50 of combined Tet and Drol was 1.66±0.41 μg/ml with the reversal effect markedly increased to 12.9. Neither 0.62 μg/ml Tet nor 1.94 μg/ml Drol could induce apoptosis of K562/A02 cells. Conclusion Mutidrug resistance(MDR) can be partially reversed by Tet or Drol, of which the combination shows a great synergistic reversal effect. The mechanism of Tet and Drol reversing multidrug resistance is not correlated with the apoptosis of K562/A02 cells.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2002年第6期526-528,共3页
Chinese Journal of Oncology
基金
国家自然科学基金资助项目 (3 9970 83 2 )
