摘要
目的 观察人脐静脉血管内皮细胞对增生性瘢痕成纤维细胞增殖活性的影响。方法收集培养的人脐静脉血管内皮细胞上清条件培养液 ,加入培养的增生性瘢痕成纤维细胞之中 ,采用MTT法检测增生性瘢痕成纤维细胞增殖率 ,利用流式细胞仪分析细胞周期。结果 加入内皮细胞上清液的增生性瘢痕成纤维细胞增殖比对照组明显增加 ,且S期比例增高。结论 在瘢痕形成的过程中 ,内皮细胞被激活 ,激活的内皮细胞可能分泌某些活性物质 ,促进成纤维细胞增殖 ,刺激其进入细胞DNA合成期S期。
Objective To investigate the influence of the endothelial cells on the scar-derived fibroblasts in the hypertropic scar tissue. Methods The endothelial cell from the human umbilical vein was cultured and its supernatant was then collected. Thereafter, it was mixed with the pre-cultured scar-derived fibroblasts from the hypertropic scar tissue. The fibroblasts were analyzed with a flow cytometer in a MTT-assay method to evaluate the cell proliferation and its division cycle. Results The endothelial cells were significantly increasing the scar-derived fibroblast proliferation (P<0.05), compared with the control. The proportion of the fibroblasts in the S-stage of cell cycle was also quite high. Conclusion The endothelial cells may secrete some active products, which may enhance the proliferation of the scar-derived fibroblasts and stimulate the fibroblasts into the S-stage of cell generation cycle.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2002年第6期338-340,共3页
Chinese Journal of Plastic Surgery
基金
教育部 1997年留学回国人员启动基金资助
