摘要
采用AB PAS染色法显微观察和分析人工养殖 3龄栉孔扇贝 (Chlamysfarreri,壳长 4 0~ 5 0mm)外套膜和鳃粘液细胞类型与分布规律。结果表明 ,粘液细胞分为 4种类型 ,Ⅰ型呈红色 ,Ⅱ型呈蓝色 ,Ⅲ型呈紫红色 ,Ⅳ型呈蓝紫色。不同部位 ,粘液细胞类型、密度及形态不同 ,这与其功能密切相关。外套膜边缘膜及生壳突起内、外表皮粘液细胞均以Ⅱ型为主 ,密度较小 ,形态主要为梨形、杯形、圆形 ,并多为大型细胞 ;中央膜内表皮以Ⅰ型和Ⅲ型较多 ,形态多为圆形或不规则形 ,外表皮只有Ⅱ型细胞分布 ;鳃轴表皮粘液细胞以Ⅰ型和Ⅲ型为多 ,形态为圆形、杯形或不规则形 ;鳃丝表皮粘液细胞多为Ⅱ型和Ⅳ型 ,形态多为圆形 ,少量为杯形 ;外套膜感觉突起和缘膜突起的表皮中无明显着色的粘液细胞 。
Mucous cells in the mantle and gill of Chlamys farreri (body length 40-50 mm)were observed and analyzed by AB PAS. Based on the observation the mucous cells are divided into four types :type Ⅰ,pure red ; typeⅡ,pure blue; type Ⅲ, purple reddish ;type Ⅳ , purple bluish . In different part, the mucous cells have different types, densities and shapes, which are closely related to the cells' function. The mucous cells in the inner and outer epithelia of mantle margin and shell secreting process (outer mantle fold)are mainly type Ⅱ at a low density, and mainly in 'pear', 'cup' and 'circle' shapes. The mucous cells in the inner epithelia of mantle central region are mainly types Ⅰand Ⅲ, but in the outer epithelia there is only typeⅡ.The mucous cells in the epithelia of gill axis are mainly types Ⅰ and Ⅲ, mainly in 'circle', 'cup' and irregular shapes .The mucous cells in the epithelia of gill filaments are mainly types Ⅱ and Ⅳ, mainly in 'circle' shape . The epithelia of mantle feeling process and edging process are filled with plenty of brown particulates.
出处
《中国水产科学》
CAS
CSCD
北大核心
2002年第4期315-317,W006,共4页
Journal of Fishery Sciences of China
基金
国家"九七三"计划资助项目 (G19990 12 0 0 5 )
关键词
栉孔扇贝
外套膜
鳃
粘液细胞
Chlamys farreri
mantle
gill
mucous cells