摘要
目的 研究全脑缺血诱导海马组织信号转导与转录激活子 - 3(STAT3)的激活调控。方法 采用SD雄性大鼠四动脉结扎建立的全脑缺血模型 ,以及腹腔注射给药的方法。运用免疫印迹和电泳迁移率改变实验检测蛋白酪氨酸激酶和蛋白酪氨酸磷酸化抑制剂对海马核抽提物STAT3的磷酸化水平及DNA结合活性的影响。结果 缺血前 2 0min腹腔注射染料木黄酮能明显抑制全脑缺血导致核内STAT3磷酸化水平及DNA结合活性的增高 ;而蛋白磷酸酶抑制剂矾酸钠则能明显促进两者的增加。
Objective To investigate the regulation of activation of STAT3 and DNA binding activity in hppocampus after global brain ischemia. Methods Four-vessel occlusion ischemia model of SD rats was used in this study. The changes of p-STAT3 and DNA binding activity after pretreatments with inhibitors of protein tyrosine kinase and protein tyrosine phosphatase in nuclear extracts were assessed by immunoblotting and electrophoretic mobility shift assay (EMSA). Results The p-STAT3 and the DNA binding activity were obviously inhibited by genistein, but markedly promoted by sodium orthovanadate, when these drugs were administered i.p. 20 minutes before occlusion. Conclusion The increments of p-STAT3 and DNA binding activity following global cerebral ischemia may be regulated by protein tyrosine kinase and protein tyrosine phosphatase as well.
出处
《徐州医学院学报》
CAS
2002年第6期485-489,共5页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学基金资助项目 (No .30 0 70 182 )
