摘要
用维生素 C(Vc)含量为0～1200mg·kg^(-1)的精制饲料,连续投喂异育银鲫8周后,测定异育银鲫生长速度和非特异性免疫指标。结果表明,在饲料Vc含量达150mg·kg^(-1)时日增重率最大,进一步提高饲料Vc含量,日增重率没有显著变化;饲料Vc含量为75mg·kg^(-1)时头肾指数显著提高,而脾指数不受饲料Vc含量的影响;头肾细胞吞噬活性和A花环率均在Vc含量为300mg·kg^(-1)时达最高水平;而血清溶菌酶活性和总补体活性分别在饲料Vc为300mg·kg^(-1)和600mg·kg^(-1)时达最高值。上述结果表明饲料Vc可显著提高异育银鲫免疫力。鉴于Vc的免疫促进作用,建议在疾病多发季节饲料Vc添加量提高到300mg·kg^(-1)。
Allogynogenetic silver crucian carp ( Carassius auratus gibelio(?)× Cyprinus carpio (?) ) were fed with purified diets containing 0 to 1200 mg·kg-1 of vitamin C (Vc) in the form of ascorbate polyphosphate for 8 weeks. The aim of this experiment was to determine the Vc requirement of allogynogenetic silver crucian carp with growth and non-specific immunity. The results showed that the highest growth rate was reached at dietary Vc level of 150 mg·kg-1, while increasing dietary Vc level from 150mg·kg-1 to 1200 mg·kg-1 gave no significant change in growth rate; The dietary Vc level of 75 mg·kg-1 markedly enhanced head kidney index, however, no difference was seen in spleen index when the dietary Vc level increased; phagocytic rate and A rosette of head kidney cell increased significantly when dietary Vc level was at 300mg·kg-1, but even more Vc amount led to no improvement in immune function of head kidney cells; The total activities of sera complement and lysozyme reached the highest values at the dietary Vc level of 600 mg·kg-1 and 300 mg·kg-1, respectively. These data indicated that increasing dietary Vc level resulted in significant increase of non-specific immunity . For the immunomodulatory effect of Vc, it is suggested that Vc content up to 300 mg·kg-1 be added to the diet of allogynogenetic silver crucian carp in the disease-prevalent seasons.
出处
《水产学报》
CAS
CSCD
北大核心
2002年第4期351-356,共6页
Journal of Fisheries of China
基金
苏州大学青年教师基金(Q3114810)
苏州市农业科技发展基金(SNZ-0117)
关键词
生长
非特异性免疫力
异育银鲫
维生素C
需要量
allogynogenetic silver crucian carp
vitamin C
growth
non-specific immunity
