摘要
目的研究胸腺肽β4(Tβ4)对肥大细胞的致敏作用及可能的机制。方法将不同浓度的Tβ4作用于肥大细胞株RBL-2H3,检测肥大细胞释放的β氨基己糖苷酶。用丝裂原活化蛋白激酶(MAPK)信号通路特异性抑制剂10μmol·L^(-1)U0126或抗过敏剂5 mmol·L^(-1)色甘酸钠分别联合4 pmol·L^(-1)Tβ4作用于小鼠肠黏膜肥大细胞,检测是否可以阻断Tβ4对肥大细胞β氨基己糖苷酶的释放作用。结果随着Tβ4浓度的增加对肥大细胞的脱颗粒效应逐渐增加,在4 pmol·L^(-1)时达到峰值。同样,4 pmol·L^(-1)的Tβ4可显著引起肠道肥大细胞的过敏反应,U0126和色甘酸钠可以抑制Tβ4的致敏效应。结论 Tβ4可通过MAPK信号通路致敏肠黏膜肥大细胞,但可被U0126和色甘酸钠阻断。这为Tβ4的药物开发和应用提供参考依据。
Objective To investigate the effect of thymosin β4( Tβ4)activating mast cells and its possible mechanism. Methods To observe the activation of mast cells,mast cells RBL-2 H3 and mouse intestinal mucosae were stimulated by Tβ4 at various concentrations,then the release of β-hexosaminidase was determined. In addition,mitogen-activated protein kinase( MAPK) specific inhibitor U0126( 10 μmol·L-1)or disodium cromoglycate( 5 mmol·L-1) was used to block the activation of mice intestinal mucosal mast cells induced by Tβ4( 4 pmol·L-1). Results In this study,we found that the degranulation effect of mast cells was gradually increased with the dose of Tβ4,peaking at 4 pmol·L-1. Similarly,Tβ4( 4 pmol·L-1) caused the sensitivity of intestinal mast cells. However,the effect of Tβ4 on intestinal mast cells could be inhibited by U0126 and disodium cromoglycate. Conclusion Tβ4 sensitizes the intestinal mucosal mast cells through MAPK signaling pathway,which can be blocked by disodium cromoglycate. This will provide a reference for the drug development and administration of Tβ4.
作者
陈婧
张彤彤
单晶
赵聪
潘逼然
邢晨
郭元彪
孙晓滨
CHEN Jing;ZHANG Tong-tong;SHAN Jing;ZHAO Cong;PAN Bi-ran;XING Chen;GUO Yuan-biao;SUN Xiao-bin(Department of Digestive,SouthwestMedical University,Luzhou 646000,Sichuan Province,China;Medical Research Center,The Third People’s Hospital of Chengdu,Chengdu 610031,China;Department of Digestive,The Third People’s Hospital of Chengdu,Chengdu 610031,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2019年第3期265-267,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家自然基金面上项目(81270465)
四川省科技厅课题资助项目(2014JY0017
2015FZ0072)
成都市科技局课题资助项目(2015-HM0-00377-SF)
关键词
胸腺肽β4
肥大细胞
过敏反应
丝裂原活化蛋白激酶
肠黏膜
thymosin β4
mast cell
allergic reaction
mitogen-activated protein kinase
intestinal mucosae
