摘要
目的 通过荧光定量PCR技术检测阴道内解脲脲原体感染状况 ,用以指导临床正确治疗。方法 对门诊的阴道炎患者 ,采用特制密封棉拭子宫颈管内取样 ,然后采用重量差减法 ,求出分泌物的重量 ,采用荧光定量PCR测定解脲脲原体。FQ -PCRUu的拷贝数 /g分泌物 ,与临床患者不同的症状体征作对照研究。结果 解脲脲原体拷贝数 /g≥ 1× 10 6的患者 ,82 9%具有典型阴道炎症状 ,71 9%具有阴道炎体征。拷贝数 /g <1× 10 6的患者 ,症状体征均不明显 。
Objective: To understand the infection of vaginal Uu detedted by fluorescent quantitative PCR(FQ-PCR) in order to guide the correct clinical treatment. Method: We use sealed cotton swab to sample in cervix.We get the weight of secretion by differential subtract method then to detect the Uu by FQ-PCR.We undergo matched control study according to the copy number of Uu /gram scretion and patients with different symptoms. Result: When Uu copy number/gram reaches ≥1×10 6 almost 82.9% have typical symptoms of vaginitis,71.9% have physical sign of vaginitis.When Uu copy number/gram reaches <1×10 6, the patient have no obvious symptoms and physical sign,there was no significant difference between the above.
出处
《中国优生与遗传杂志》
2002年第5期30-31,共2页
Chinese Journal of Birth Health & Heredity
