摘要
【目的】苹果果实轮纹病是我国苹果的重要病害之一,研究果实相关抗病性基因对苹果育种有重要意义。【方法】以苹果(Malus domestica)‘红玉’ב金冠’杂交实生树为材料,对果实接种5个轮纹病菌(Botryosphaeria dothidea)后调查其潜伏期、病斑大小、发病率、病斑扩展速率等指标,运用已有的SSR图谱和苹果基因组注释信息,对苹果果实轮纹病进行QTL(Quantitative Trait Loci)分析并对苹果轮纹病抗病基因进行预测。【结果】得到与苹果果实轮纹病抗病性相关的QTLs共29个,分布在LG5、LG10、LG12、LG15、LG17等5个连锁群上。在QTLs位点的23个侧翼SSR标记中,有18个可以从苹果基因组上相对应的contig位置截取到6个区间的序列信息共35 Mb,共含5 620个基因。最终根据苹果基因注释预测得到与抗病相关的基因107个,其中与抗病蛋白相关的基因60个,编码亮氨酸结构域的基因3个;编码防御酶的基因41个;编码与病程相关蛋白有关的基因3个。【结论】预测到与苹果轮纹病相关的基因107个,为进一步进行抗病性基因挖掘提供数据。
【Objective】Fruit ring rot(Botryosphaeria dothidea)is one of the important diseases for apples,so it is important for fruit breeders to study resistant genes of apple to fruit ring rot.【Method】In the study,five different isolates of pathogen(Botryosphaeria dothidea) were used to inoculate apple fruits derived from ‘Jonathan'בGolden Delicious' (Malus domestica),and latent period,diameter,infectionrate,and expanding rate of the spot were investigated respectively.QTL analysis and related genes prediction were conducted based on the SSR linkage maps and the annotation of apple genome.【Result】29QTLs related to the resistance of Malus domestica to apple fruit ring rot were mapped,which located in LG5,LG10,LG 12,LG15,and LG17.In 23 SSR markers flanking loci of QTLs,18 markers can beclipped to 6 fragments from the corresponding contig locations of apple genome that contains 35 Mb sequence information and 5 620 genes.107 disease-related genes were obtained based on the annotation of apple genome.In these genes,60 genes encode disease resistance protein,3 genes encode NBS-LRR-domains protein,41 genes encode defensive enzyme(PAL,POD and SOD) and 3 genes encode pathogenesis-related proteins.【Conclusion】107 disease-related genes were obtained.This study would provide datafor further excavating the resistant genes in apple trees.
出处
《果树学报》
CAS
CSCD
北大核心
2014年第5期793-800,共8页
Journal of Fruit Science
基金
国家863课题(2011AA001204)
农业部园艺作物营养与生理重点实验室
北京市果树逆境生理与分子生物学重点实验室
关键词
苹果
苹果轮纹病
QTL
SSR
抗病基因
Malus domestica
Botryosphaeria dothidea
Quantitative trait loci
Simple sequence repeat
Resistance gene
