摘要
目的 利用Ⅳ型胶原酶构建大鼠脑出血模型 ,并研究血肿形成速度和血肿形成大小的影响因素。方法 使用健康雄性 2 0 0～ 2 5 0gWistar大鼠 ,随机分组 ,分别脑内尾状核注射不同容积Ⅳ型胶原酶 肝素生理盐水溶液、单纯Ⅳ型胶原酶生理盐水溶液、单纯生理盐水 ,于术后 6h、12h、2 4h、3d、7d分别观察冰冻切片连续切片血肿大小。结果 单纯生理盐水注射仅引起 12h内的针道渗血 ,单纯胶原酶生理盐水注射 3d可以形成直径 3mm左右的血肿 ,Ⅳ型胶原酶 肝素生理盐水注射 2 4h可形成直径 3mm左右的血肿。结论 Ⅳ型胶原酶 肝素生理盐水注射可以建立理想稳定的脑出血动物模型 。
Objective To establish a cerebral hemorrhage model in rats by local injection of type Ⅳcollagenase, and explore the factors affecting the speed and volume of hematoma formation Methods A total of 150 healthy male Wistar rats weighing from 200 to 250 g were randomly divided into 3 groups, control, collagenase, and collagenase/heparin groups Animals in those groups received injection of saline, collagenase (0 2 U/μl), and collagenase (same dose) and heparin (2 U/μl) with different volume respectively at the caudate nucleus The volume of the hematoma in the rats ( n =6 at each time point) was observed 6, 12, and 24 h, and 3, 7d after the injection Results Permeation of blood was found in 12 h after injection in control group Hematoma about 3 mm in diameter was found in 3 d after injection in collagenase group, while in collagenase/heparin group, it was found in 24 h Conclusion Cerebral hemorrhage model established by local injection of collagenase/heparin in saline solution is ideal and reliable, and the size of hematoma is in correlation with the volume of solutions injected into the brain
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第12期1394-1395,共2页
Journal of Third Military Medical University
基金
第三军医大学基金资助项目 ( 2 0 0 2 )
关键词
Ⅳ型胶原酶
大鼠
脑出血模型
collagenase
cerebral hemorrhage
animal model
