摘要
作者将副流感病毒3型(PIV-3)单克隆抗体(McAb)用于APAAP桥联酶标技术快速检测患儿PIV-3抗原100例,并与IFA技术及血凝抑制试验(HAI)作了比较,获得满意效果。其与IFA的阳性和阴性符合率均>96％。与HAI的阳性符合率为88％,阴性符合率为100％。由于呼吸道细胞内很少含有碱性磷酸酶,因此没有内源酶的干扰。另外,此技术不需荧光显微镜,一般光学镜下染色格外清晰,两小时可得到准确结果。
In the present study, using APAAP enzyme sign, monoclonal antibodies against parainfluenza virus type 3. ( PIV-3) were used for rapid detection of PIV-3 antigens in 100 patients. The results indicated that the present method was superior to IFA technique and double serUmftest. The positive and negative agreement rates of APAAP and IFA were more than 96% and the positive and negative agreement rates of APAAP and double serum test were 88% and 100%, respectively. The results were clearly visible under optical microscope as interference by endogenous enzyme may be avoided owing to the fact that little alkaline phosphatase is present in respiratory cells. This technique could produce accurate report only within two hours.
关键词
副流感病毒
单克隆抗体
诊断
parainfluenza virus type 3
antibody, monoclonal
APAAP bridge enzyme labelled technique
IFA
