摘要
色氨酸是一种具有强荧光特性的人体必需氨基酸,具有手性特性,分为L-色氨酸(L-Trp)和D-色氨酸(D-Trp)两种对映体。本文研究发现L-/D-色氨酸的本源荧光在pH为6.24和8.67的磷酸盐缓冲溶液中分别被金属离子Pd(Ⅱ)和Cu(Ⅱ)所猝灭,同时猝灭体系的荧光可在草甘膦(Gly)的加入后得到恢复。因此基于L-/D-色氨酸的荧光'关-开'的机制,建立了分析草甘膦的方法。在最优条件下,Trp-Pd(Ⅱ)体系检测草甘膦的范围为0.075~0.70μg·mL-1,而Trp–Cu(Ⅱ)体系检测草甘膦的范围为0.025~1.0μg·mL-1,检出限分别可达15.21和8.61ng·mL-1。同时文章研究了共存物质的影响,讨论了体系反应机理。
Tryptophan is an essential amino acid with strong fluorescence and its two enantiomers are L-Tryptophan(L-Trp)and D-Tryptophan(D-Trp).This study found that the fluorescence of L-/D-Trp in pH 6.24 and 8.67 phosphate buffer solution could be quenched by Pd(Ⅱ)and Cu(Ⅱ),respectively,and then recovered by adding glyphosate(Gly).Based on the'turn-offon'fluorescent property,a convenient method for analysis of Gly was proposed by using L-/D-Trp.Under optimum conditions,the detecting range of Gly in Trp-Pd(Ⅱ)system and Trp-Cu(Ⅱ)system were 0.075~0.70 and 0.025~1.0μg·mL-1,and the limit of detection were 15.21 and 8.61ng·mL-1 respectively.The effect of coexisting substances and the reaction mechanism were both investigated.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2016年第S1期357-358,共2页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(21175015
21475014)资助
