摘要
目的对化学发光法与聚合酶链反应(PCR)联合检测孕妇单纯疱疹病毒(HSV)的感染率进行分析,为临床诊断提供依据。方法采集该院孕期常规建卡的孕妇血清,用化学发光法检测血清HSV IgM与IgG抗体水平;对其中任一项结果阳性者取宫颈分泌物进行HSV DNA定性检测。结果HSV1DNA阳性率为0.5%(7/1 422),HSV2DNA阳性率为1.1%(16/1422)。HSV IgM和IgG同时阳性者HSV1DNA阳性率为0.4%(4/1 008),HSV2DNA阳性率为0.6%(6/1 008);仅HSV IgM阳性者HSV1DNA阳性率为0.8%(1/130),HSV2DNA阳性率为3.1%(4/130);仅HSV IgG阳性者HSV1DNA阳性率为0.7%(2/284),HSV2DNA阳性率为2.1%(6/284)。3种情况HSV抗体阳性者HSV1DNA阳性率比较差异无统计学意义(P>0.05),而HSV2DNA阳性率比较差异有统计学意义(P<0.05)。结论在孕期对HSV抗体进行常规检查,对抗体阳性者进行DNA检测,能提高诊断准确率。尽量做到早期筛查、早期发现、及早对孕妇进行治疗。
Objective Detect the infection rate of herpes simplex virus(HSV)by jointly using chemiluminescence assay and PCR,and provides reference for clinical diagnosis.Methods The serum samples were collected from the pregnant women who had routine examination records in the hospital.Chemiluminescence assay was used to detect HSV IgM and IgG in those samples.Cervical secretions were collected from pregnant women with positive results and qualitatively tested for HSV DNA.Results The positive rate of HSV1 DNA was 0.5%(7/1 422),the positive rate of HSV2 DNA was 1.1%(16/1 422).For pregnant women whose HSV IgM and IgG were both positive,positive rate of HSV1 DNA was 0.4%(4/1 008)and that of HSV2 DNA was 0.6%(6/1 008);for those who only had HSV IgM positive,the positive rate of HSV1 DNA was 0.8%(1/130),and that of HSV2 DNA was 3.1%(4/130);for those who only had HSV IgG positive,the positive rate of HSV1 DNA was 0.7%(2/284),that of HSV2 DNA was 2.1%(6/284).Among those three HSV antibody positive cases,the difference in HSV1 DNA positive rate was not statistically significant(P>0.05),while the difference in HSV2 DNA positive rate was statistically significant(P<0.05).Conclusion The test of HSV antibodies during pregnancy can be used as a routine test,and HSV DNA test can be used as further test for those with HSV antibody positive,which could improve the accuracy of diagnosis.Early screening,detection,and treatment are important for pregnant women with HSV infection.
出处
《国际检验医学杂志》
CAS
2016年第2期157-158,共2页
International Journal of Laboratory Medicine
关键词
化学发光测定法
聚合酶链反应
单纯疱疹病毒属/分离和提纯
孕妇
chemiluminescent measurements
polymerase chain reaction
simplexvirus/isolation & purification
pregnant women
