摘要
ATP合酶是光合作用中光合磷酸化和呼吸作用中氧化磷酸化反应的关键酶,影响着细胞生命活动所需的ATP的产生。为研究ATP合酶在苔藓植物中的生物学功能,本研究克隆得到砂藓ATP合酶Ⅱ亚基基因,命名为Rcatp G。生物信息学分析表明,砂藓的c DNA序列长为1 220 bp,其中开放阅读框(ORF)为756 bp,编码251个氨基酸的多肽序列;预测分子质量为27.48 k D,等电点为9.20,含有ATP-synt_B保守结构域;推测Rcatp G蛋白为不稳定蛋白,不属于跨膜蛋白且不存在信号肽。系统发育分析显示,Rcatp G蛋白与小立碗藓atp G蛋白亲缘关系最近。实时荧光定量PCR分析显示,Rcatp G基因在复水过程和脱水胁迫处理中均能表达。因此,推测Rcatp G基因在砂藓的复水过程和脱水胁迫处理中起着一定的作用。
ATP synthase, one of the key enzymes in photosynthesis of photosynthetic phosphorylation and in respiration of oxidative phosphorylation, is essential for research of molecular basis about the growth of cells in Racomitrium canescens. This paper aimed to study the role of ATP synthase under drought tolerance of Racomitrium canescens. We cloned and sequenced Rcatp G gene with RT-PCR technique and performed bioinformatic analysis.The expression levels of Rcatp G in both rehydration and dehydration treatment to Racomitrium canescens were detected by quantitative RT-PCR. The full length of this gene was 1 220 bp, and contained a 756 bp open reading frame which encoded a protein containing 251 amino acids. Bioinformatic analysis showed that the Rcatp G was predicted as an unstable protein with a function domain ATP-synt_B, with molecular weight of 27.48 k D and isoelectric point of 9.20. The protein was also predicted that it is not a transmembrane protein and containing no signal peptides. Phylogenetic analysis indicated that this gene has the closest genetic relationship with the homolog gene in Physcomitrella patens. The quantitative RT-PCR results suggested that the expression of Rcatp G gene could be induced in both rehydration and dehydration treatment to Racomitrium canescens. It suggested that this gene might play an important role in both rehydration and dehydration processes.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第7期1435-1442,共8页
Genomics and Applied Biology
基金
黑龙江省自然科学基金重点项目(ZD201408)
国家自然科学基金项目(31070180
31270254)共同资助
关键词
砂藓
ATP合酶
基因克隆
生物信息学分析
实时荧光定量
Racomitrium canescens,ATP synthase,Gene cloning,Bioinformatic analysis,Quantitative real-time PCR
