摘要
本研究通过对皮革肾岛衣(Nephrmopsis pallescens)地衣型真菌转录组数据的分析,首次克隆得到一种高度还原型聚酮合酶(Highly reducing PKS)基因全长,并对该基因进行生物信息学分析,并检测该基因在不同培养基上的表达量。该基因cDNA全长7 491 bp (命名为NpPKS1),可编码2 496个氨基酸,是一种稳定存在于细胞质基质中的非分泌蛋白;结构域与其他真菌的洛伐他汀九酮合成酶(LNKS)结构域极为相似,且聚类分析与其他真菌的洛伐他汀九酮合成酶(LNKS)聚为一类;在以葡萄糖和麦芽糖作为碳源的情况下,番茄浸粉、牛肉浸粉、酪蛋白胨等作为氮源可强烈刺激NpPKS1基因的表达,而胰蛋白胨对该基因的表达存在一定程度的抑制效应。本研究为皮革肾岛衣地衣型真菌中的基因资源利用、聚酮化合物异源表达和其合成机制的研究奠定了基础。
The present study cloned the full length of a highly reducing PKS gene through analyzing the transcriptome data of Lichenized-fungi of Nephrmopsis pallescens,simultaneously took a bioinformatics analysis to this gene,and tested the gene expression level in different medium.The cDNA of this gene had a total length of 7 491 bp(named NpPKS1),could encode 2 496 amino acids,was a stable non-secretory protein locating in cytoplasmic matrix.The domain was very similar to that of lovastatin nonaketide synthase(LNKS)of other fungi,and cluster analysis was the same as that of LNKS of other fungi.With glucose and maltose as carbon sources,tomato extract,beef extract and casein peptone as nitrogen sources could strongly stimulate the expression of NpPKS1 gene.Inversely,tryptone had certain inhibitory effect to the gene expression.The present study could lay a foundation for the study of gene resource utilization,heterologous expression and the biosynthesis mechanism of polyketide in Lichenized-fungi of Nephrmopsis pallescens.
作者
原晓龙
陈剑
华梅
王娟
王毅
Yuan Xiaolong;Chen Jian;Hua Mei;Wang Juan;Wang Yi(Yunnan Academy of Forestry,Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants,Yunnan Provincial Key Laboratory of Conservation and Breeding of Rare and Endangered Forest Plants,State Forestry Administration,Kunming,650204)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第4期1608-1614,共7页
Genomics and Applied Biology
基金
国家自然科学基金项目(31860177)
云南省应用基础研究计划面上项目(2016FB055)
云南省对外科技合作计划项目(2015IA004)共同资助
关键词
皮革肾岛衣
聚酮合成酶
生物信息学
基因表达
Nephrmopsis pallescens
Polyketide synthase
Bioinformatics
Gene expression
