摘要
为了解向日葵锈菌(Puccinia helianthi)冬孢子及锈孢子的生物学特性,对这两种类型孢子最适萌发条件进行了筛选研究。结果表明,采集的新鲜冬孢子在室温下存放超过180 d才可萌发。存放于-20℃萌发效果较好,180 d萌发率可达到39.45%,420 d时,冬孢子活性基本丧失。冬孢子在水琼脂培养基上于15℃12 h即可萌发,48 h后达到萌发高峰,萌发率为31.89%;锈孢子在水琼脂培养基上于20℃2 h开始萌发,24 h后萌发率最高,萌发率为54.33%;水琼脂培养基浓度对冬孢子的萌发无影响,0.50%琼脂培养基最适宜锈孢子的萌发;温度15℃、pH 6的条件有利于冬孢子萌发;20℃、 pH 7是锈孢子萌发的最适宜温度和pH值;光照对冬孢子萌发没有影响,但是对锈孢子萌发有促进作用。10 g/L低浓度的叶片浸出液可以促进两种孢子的萌发,高浓度抑制萌发。
To understand the biological characteristics of teliospores and aeciospores of P.helianthi,this study investigated the optimal germination conditions for these two types of spores.The results revealed that the collected fresh teliospore could only germinate after being stored at room temperature for more than 180 days.Storage at−20℃had a better germination effect,with a germination rate of 39.45%after 180 days,however by 420 days,the activity of teliospores was basically lost.Teliospores could germinate on water agar medium at 15℃for 12 hours,reaching a peak germination rate of 31.89%after 48 hours.Aeciospores began to germinate on water agar medium at 20℃within 2 hours,and reached the highest(54.33%)after 24 hours.The concentration of water agar medium had no effect on the germination of teliospores,but 0.50%agar medium was most suitable for the germination of aeciospores.The conditions of 15℃and pH 6 were favorable for the germination of teliospores;20℃and pH 7 were the most suitable for the germination of aeciospores.Light had no effect on the germination of teliospores but promoted the germination of aeciospores.A low concentration(10 g/L)of leaf extract could promote the germination of both types of spores,while a high concentration inhibited them.
作者
王佳运
路妍
孔祥久
景岚
WANG Jia-yun;LU Yan;KONG Xiang-jiu;JING Lan(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China)
出处
《中国油料作物学报》
北大核心
2025年第1期204-210,共7页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(32160642)
内蒙古自治区高等学校创新团队发展计划(NMGIRT2320)
内蒙古自治区研究生教育教学改革项目(JGCG2023070)。
关键词
向日葵锈菌
冬孢子
锈孢子
萌发条件
Puccinia helianthi
teliospore
aeciospore
germination conditions
