摘要
假单胞菌属(Pseudomonas spp.)是一类重要的食源性致病菌和腐败菌。噬菌体裂解酶具有高效裂解活性和靶向性等特征,可作为有效控制假单胞菌属细菌的新型抗菌剂。随着组学技术的高速发展,大量噬菌体的基因组信息得到破译,为噬菌体裂解酶生物信息的挖掘提供了数据资源。本研究基于NCBI等数据库,深入挖掘假单胞菌噬菌体裂解酶的理化参数、核心结构域、三维结构等生物信息。结果表明,共筛选获得了81个噬菌体裂解酶序列,此类裂解酶大多为亲水蛋白(80/81),均具有较高的等电点,蛋白总体带正电。在所筛选的裂解酶序列中,共发掘9个保守结构域,分别为Lyz-like super family、yz_endolysin_autolysin、Muramidase、PGRP super family、NlpC/P60、LT-like、Phage_lysis Superfamily、PG_binding_1、PG_binding_3。同源建模分析发现,裂解酶末端α-螺旋上均分布一定的正电荷基团,预测该结构可能赋予其穿透假单胞菌外膜的功能。本研究为假单胞菌噬菌体裂解酶的设计、改造和生产提供理论支撑,为假单胞菌的靶向防控提供重要的数据基础。
Pseudomonas spp.is a kind of crucial foodborne pathogenic bacteria and spoilage bacteria.Phage lysins have the characteristics of high cleavage activity and targeting,and can be employed as a novel antibacterial agent to successfully control Pseudomonas bacteria.Because of the rapid advancement of genomics technology,a huge number of bacteriophage genome information has been deciphered,providing data resources for the excavation of phage lysins biological information.In this study,in-depth excavation of Pseudomonas phage lysins physical and chemical parameters,core domain,three-dimensional structure and other biological information was performed using NCBI and other databases.The results showed that a total of 81 phage lysin sequences were obtained.The majority of these lysins were hydrophilic proteins with high isoelectric point and positively charged proteins.A total of 9 conserved domains were found in the selected lysin sequences,being Lyz-like super family,lyz_endolysin_autolysin,Muramidase,PGRP super family,NlpC/P60,LT-like,Phage_lysis Superfamily,PG_binding_1,PG_binding_3.Homologous modeling analysis has found that a specific positive charge group was distributed on the α-helix at the end of the lysins,predicting that it would have the role of piercing the Pseudomonas outer membrane.This study provided theoretical support for the design,transformation and production of Pseudomonas phage lysins,as well as an important data foundation for Pseudomonas-targeted prevention and control.
作者
惠潇然
黄振华
刘静
吴倩
许天明
段为旦
潘迎捷
赵勇
张昭寰
HUI Xiao-ran;HUANG Zhen-hua;LIU Jing;WU Qian;XU Tian-ming;DUAN Wei-dan;PAN Ying-jie;ZHAO Yong;ZHANG Zhao-huan(Coll.of Food Sci.&Tech.,Shanghai Ocean Uni.,Shanghai 201306;Lab.of Qual.&Safety Risk Assess′t for Aquatic Prod.on Storage&Preserv′n(Shanghai),Minist.of Agric.&Rural Affairs,Shanghai 201306;Shanghai Engin.Res.Ctr.of Aqua-Prod.Process.&Preserv′n,Shanghai 201306)
出处
《微生物学杂志》
北大核心
2025年第1期14-23,共10页
Journal of Microbiology
基金
国家自然科学基金面上项目(31972188)
上海市自然科学基金项目(22ZR1427500)
上海市优秀学术带头人计划资助项目(21XD1401200)。
