摘要
PCR扩增分析中国华北型优良地方猪种莱芜黑猪和产仔数与其差异较大的大约克夏 ,以及它们的杂交后代大莱猪在卵泡刺激素 (FSH) β亚基基因结构区的多态性。通过测序发现莱芜黑猪在该区存在插入突变 ,插入片段长度为 2 75bp ,位于已发表的猪FSHβ亚基基因全序列的 +80 9与 +810碱基之间 ,插入片段中存在一个RNA聚合酶Ⅲ启动子及AluI内切酶识别位点 ,末端有 17个腺苷酸的poly(A) ,所以该片段可能为Alu成分。对FSHβ亚基基因插入片段进行BamHI多态性分析 ,发现本研究所检测样品中不存在BamHI多态性。把FSHβ亚基基因作为控制猪产仔数主效基因的候选基因与产仔数进行连锁分析 ,证明FSHβ基因座位在莱芜猪种中与控制猪产仔数的主效基因紧密连锁 ,优势基因AA纯合子比BB纯合子母猪平均每胎多产仔 1.2头。因莱芜猪与太湖猪插入序列的主要差异是末端的poly(A)长短不同 ,故推测该插入片段末端的poly(A)结构亦可能影响猪的产仔数。
FSHβ subunit gene in Laiwu pigs--one excellent local pig breed in North China, Large white pigs and Dalai pigs were amplified by PCR, cloned and sequenced. Sequences analysis showed that there was an inserted mutation in FSHβ gene of Laiwu pigs. The length of the inserted fragment was 275bp, between +809bp and +810bp of the published sequences (D00621). According to the poly (A) with 17 adenines, the RNA polymerase III promoter structure and Alu I restriction enzyme site in the inserted fragment, it should be regarded as an Alu element. The major difference of the inserted fragment between Laiwu and Taihu breeds was originated from the length of poly (A) end, so it was assumed that the poly (A) structure in the inserted fragment should influence the pig litter size. Because Laiwu pigs with genotype AA had 1.2 litter size more than those with genotype BB , it could be concluded that FSHβ subunit gene was related with pig litter size traits or linked with the genes of which, and the poly(A) structure in this Alu element played an critical role.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2002年第4期403-408,共6页
Journal of Shandong Agricultural University：Natural Science Edition
