摘要
本实验应用放射配体受体检测法Scatchard pseudoscatchard分析,证实大鼠脑胞液中存在高亲和力结合位点(HAGS)和低亲和力结合位点(LAGS)两类糖皮质激素(GC)结合位点。其解离常数(Kd)分别为(2.78±0.71)×10^(-8)mol/L和(2.12±1.00)×10^(-6)mol/L。大鼠创伤性脑水肿后6 h,伤测大脑半球脑胞液中糖皮质激素受体(GR)较健侧及正常脑胞液中GR明显减少,而Kd无显著变化。伤后6h肝胞液GR明显减少,Kd也无显著变化。根据大剂量GC治疗中枢神经系统损伤时组织局部GC浓度与GC低亲和力结合位点Kd非常接近的现象,我们认为大剂量GC治疗创伤性脑水肿的作用机理,是通过增加激-受体复合物的生成,且很有可能是通过GC的低亲和力结合位点介导而起作用。
The high-affinity glucocorticoid binding sites (HAGS) and the low-affinity glucocorticoid binding sites (LAGS) with steroid specificity were demostrated in cerebral cytosol of rats by using the radioligand binding assay. The Kd of HAGS and LAGS were (178±0.71)×l0-8mol/L and (2.12±1.06)×10-6mol/L respecitively as estimated by Scatchard and Pseudoscatchard analysis. Glucocorticoid receptors (GR) in the traumatized(left) hemisphere cytosol were decreased more significantly than those in both the control (right) hemisphere cytosol at 6h postinjury and normal brain tissue (P<0.05), but Kd of GR showed no agnificant changes. GR of liver cytosol at 6h postinjury was more markedly decreased than normal hepatic cytosol, but Kd of GR underwent no significant changes. These data demonstrate that high-dose GC used in the treatment of traumatic brain edema might maintain target-cell reactions by increasing the production of GC receptor complexes and is most likely to be mediated by LAGS.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1992年第1期1-5,共5页
Academic Journal of Second Military Medical University
关键词
脑损伤
急性
脑水肿
受体
糖皮质激
brain injuries, acute
brain edema
receptors, glucocorticoid
disease models, animal
rats
