摘要
In acidic solution, thorin (2-(2-hydroxy-3.6-disulfo-1-naph-thyl)-azo-phenylarsenic acid sodium salt) can be bound with proteinand aggregated to form large particle which displays a very strong rayleigh light scattering (RLS).The effects of pH, thorin concentration, detergent and ionic strength on binding reaction have beenstudied. The interference of coexisting substances was checked. The Scatchard plots for reactionbetween thorin and bovine serum albumin (BSA) were constructed and the association constant ofthorin-BSA was obtained, it is 5.26 x 10~5 L/mol, the maximum binding number is 7. RLS intensity iswell proportional to the concentrations of 2.0―14.0 μg/ mL for human serum albumin (HSA),1.8―14.7 μg/mL for BSA and 1.8―14.6 μg/mL for γ-globulin (γ-G). The detection limits (3σ) are54.1 ng/mL for HSA, 52.0 ng/mL for BSA and 51.8 ng/mL for γ-G, respectively. The relative standarddeviation is 2.4% for BSA, 3.2% for HSA and 4.1% for γ-G. The human serum samples were measuredsatisfactorily by using this method.
In acidic solution, thorin (2-(2-hydroxy-3.6-disulfo-1-naph-thyl)-azo-phenylarsenic acid sodium salt) can be bound with proteinand aggregated to form large particle which displays a very strong rayleigh light scattering (RLS).The effects of pH, thorin concentration, detergent and ionic strength on binding reaction have beenstudied. The interference of coexisting substances was checked. The Scatchard plots for reactionbetween thorin and bovine serum albumin (BSA) were constructed and the association constant ofthorin-BSA was obtained, it is 5.26 x 10~5 L/mol, the maximum binding number is 7. RLS intensity iswell proportional to the concentrations of 2.0―14.0 μg/ mL for human serum albumin (HSA),1.8―14.7 μg/mL for BSA and 1.8―14.6 μg/mL for γ-globulin (γ-G). The detection limits (3σ) are54.1 ng/mL for HSA, 52.0 ng/mL for BSA and 51.8 ng/mL for γ-G, respectively. The relative standarddeviation is 2.4% for BSA, 3.2% for HSA and 4.1% for γ-G. The human serum samples were measuredsatisfactorily by using this method.
基金
theNationalNaturalScienceFoundationofChina (No.2 9775 0 0 3)
