摘要
目的 NAG6、NAG 7、BRD7为最近克隆的肿瘤相关新基因 ,对它们在胃癌及癌旁组织中的表达进行检测 ,探讨这些基因在胃癌发生发展中的作用。方法 采用RT PCR、Northern杂交、点杂交方法检测 34例胃癌和癌旁组织中这些基因的表达。结果 胃癌组织中 ,有 61 .8% (2 1 / 34)NAG6基因表达缺失 ,NAG6在胃癌组织中的表达较癌旁组织显著下调 (P <0 .0 1 ) ,但NAG6表达下调与胃癌淋巴结或远处转移无明显关系 (P >0 .0 5)。NAG 7基因在胃癌和癌旁组织中表达率分别为 88.2 % (30 / 34)和 82 .3 % (2 8/ 34)。BRD7基因在胃癌及癌旁组织中均有表达。RT PCR、Northern杂交、点杂交结果均显示NAG 7、BRD7基因在胃癌与癌旁组织之间的表达差异无显著性。同时 ,点杂交也证实NAG6在胃癌组织中表达下调。结论 NAG6基因在胃癌组织中表达显著下调 ,这可能在胃癌的发生发展过程中起重要作用 ,但可能与淋巴结或远处转移无关。BRD7、NAG 7基因在胃癌中未发现表达水平改变 。
Objective NAG6, NAG 7 and BRD7 genes were novel tumor related genes identified recently. This study was designed to examine the expression of these genes in gastric cancer and matched paracancerous tissues and to investigate their roles in the occurrence and development of gastric carcinoma. Methods The expression levels of NAG6, NAG 7 and BRD7 in 34 cases of human gastric cancer and matched paracancerous tissues were analyzed by RT PCR, Northern blot analysis and Dot hybridization. Results The expression of NAG6 was absent in 61.8% gastric cancer tissues(21/34). The expression of NAG6 was significantly down regulated in gastric cancer tissues compared with that in paracancerous tissues ( P < 0.01 ). However, down regulation of NAG6 was not associated with lymph node or distance metastasis in gastric cancer( P > 0.05 ). The expression rates of NAG 7 in gastric cancer and paracancerous tissues were 88.2% (30/34) and 82.3% (28/34) respectively. BRD7 was expressed in all gastric cancers and matched paracancerous tissues. The results of RT PCR, Northern blot analysis and Dot hybridization all showed that the expressions of NAG 7 and BRD7 did not display any difference between gastric cancer and matched paracancerous tissues. Meanwhile, Dot hybridization also confirmed that NAG6 was significantly down expressed in gastric cancer. Conclusions NAG6 gene was significantly down regulated in gastric cancer tissues. The down regulation of this gene may play a key role in occurrence and development of gastric cancer, however it may be not associated with lymph node or distance metastasis. The expression levels of NAG 7 and BRD7 were not altered in gastric cancer. It seems that NAG 7 and BRD7 gene may not contribute to the occurrence and development of gastric carcinoma.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2002年第12期733-736,共4页
Chinese Journal of Digestion
基金
湖南省自然科学基金 ( 0 1JJY2 10 2 )
国家 86 3项目 ( 10 2 10 0 1 0 5)
973重大项目
