摘要
目的 探讨梗阻性黄疸形成时肝细胞凋亡与肿瘤坏死因子的关系。方法 将 72只Wistar大鼠随机分为正常组、假手术组和胆总管结扎手术组 ,以结扎胆总管 (LCD )的Wistar大鼠为模型 ,采用放射免疫法 ,测定血清中肿瘤坏死因子含量 ;末端脱氧核酸转移酶介导的末端标记法(TUNEL)检测大鼠肝细胞凋亡状态。结果 LCD术后 3、7、14、2 1d肿瘤坏死因子含量明显高于正常组和假手术组 ,并于LCD术后 7～ 14d达到高峰。LCD术后 3、7、14、2 1d肝细胞凋亡指数(HAI)亦明显高于正常组和假手术组 ,且亦于 7～ 14d达到高峰。手术组各时相肿瘤坏死因子与HAI呈正相关。结论 在Wistar大鼠梗阻性黄疸形成中肿瘤坏死因子对肝细胞凋亡起介导作用。
Objective To investingate the relationship between tumor necrosis factor-alpha (TNF-α) and hepatic cell apoptosis in obstructive jaundice.Methods 72 Wistar rats were divided into three groups:normal,sham ligating common bile duct and ligating common bile duct (LCD).Thecontent of serum TNF-α was decteted by radivimmunity and hepatic cell apoptosis by TUNEL method.Results The content of serum TNF-α and hepatic cell apoptosis index (HAI) in LCD group were higher than in nomal and sham LCD groups at 3,7,14,21 days after LCD.At the same time,both TNF-α and HAI reached peak from 7 to 14 days after LCD.Moreover,there was correlation between TNF-α and HAI.Conculsion TNF-α play a role in inducing hepatic cell apoptosis during obstructive jaundice formation and development in rats.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第1期51-52,共2页
Chinese Journal of Experimental Surgery
