摘要
法氏囊活性肽 (Bursin,BS)作为一种免疫增强剂 ,临床应用广泛 ,为了大量制备BS ,按大肠杆菌惯用密码子合成BS基因 ,并用色氨酸将 5个BS基因串联 ,克隆于质粒pUC5 7的SmaI位点 ,经测序证明序列正确后 ,以PCR扩增 ,克隆于表达载体pBV2 2 0的EcoRI,HindIII位点 ,温敏诱导表达 ,其表达水平占全菌总蛋白的 1 3 .4%。
As a immunopotentiating agent,bursin is widely used in clinic.To prepare BS largely,the gene BS was synthesized according to the preferential codons of E.coli ,and Trp was used to make five BSs contracte,BSs was cloned into SmaI site of pUC57 and sequenced.The BSs was amplified with PCR and cloned into the EcoR I and HindIII site of expression plasmid pBV220 and reached some extent expression in E.coli by inducement of temperature.The lever of expression is 13.4%.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2003年第1期29-32,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
江苏省畜禽生物制品工程技术研究中心开放课题
上海市农委重点攻关项目 [农科攻字( 2 0 0 0 )第 5_4号 ]
