摘要
目的观察母鼠青春期及孕哺期燃煤型氟中毒对仔鼠学习记忆及海马区胶质纤维酸性蛋白(GFAP)表达的影响。方法 48只断乳两周的健康清洁级SD大鼠(雌鼠32只、雄鼠16只),按体重将雌鼠随机分为对照组、低氟组、中氟组、高氟组,每组8只,其中低、中、高氟组饲料含氟量分别为24.4、47.8、106.0 mg/kg;染氟组以燃煤型氟中毒重病区原煤烘干的玉米为主要饲料喂养3个月,对照组及雄鼠食用正常饲料。染氟3个月后雌雄大鼠按2∶1合笼交配,雌鼠妊娠后继续染毒,直至仔鼠断乳,仔鼠断乳后食用正常饲料。计算仔鼠脑脏器系数,以氟离子选择电极法检测仔鼠脑氟含量,以Morris水迷宫检测30 d龄仔鼠学习记忆能力,以Western blot检测仔鼠海马区GFAP的表达水平。结果与对照组比较,各染氟组仔鼠脑脏器系数无明显差异(P>0.05);中氟组和高氟组仔鼠脑氟含量升高,差异有统计学意义(P<0.05)。与对照组比较,定位航行实验第3、4天的中氟组及高氟组仔鼠平均逃避潜伏期延长,高氟组仔鼠首次到达平台时间延长,且穿越平台次数减少,差异均有统计学意义(P<0.05)。与对照组相比,高氟组仔鼠海马区GFAP表达水平增加,差异有统计学意义(P<0.05)。结论母鼠燃煤型氟暴露可影响仔鼠学习记忆能力,其机制可能与仔鼠海马区GFAP表达水平增加有关。
Objective To observe the effect of coal-burning fluorosis during puberty and pregnancy in female rats on learning and memory and the expression of glial fibrillary acidic protein(GFAP)in hippocampus of rat offspring.Methods Forty-eight SD rats were weaned for two weeks(32 females and 16 males).According to body weight,female rats were randomly divided into control,low-fluoride,medium-fluoride and high-fluoride exposure group,eight in each,the fluoride content in feed of low,moderate and high dose group was 24.4,47.8 and 106.0 mg/kg respectively;Fluoride exposure groups were fed with the corn dried by burning coal from the endemic fluorosis area and fed for three months,the control and male rats were fed with normal diet,after three months,females and males rats were mated at a ratio of 2∶1,the fluoride exposure lasted during pregnancy and lactation,and normal diet was given for weaned rat offspring.The organ coefficient of brain of offspring was calculated,fluoride in the brain was determined by fluorine ion selective electrode method,and the learning and memory ability of 30-day-old rats were tested by Morris water maze,Western blot was used to detect the expression of GFAP in hippocampus of offspring.Results Compared with the control,no significant difference was seen in organ coefficient of brain in fluoride exposure groups(P>0.05),the contents of fluoride in the brain in moderate-fluoride and high-fluoride group significantly increased(P<0.05);Compared with the control,the average escape latency of offspring in the moderate-fluorine and high-fluorine groups at the 3 th and 4 th day of the Morris water maze test significantly prolonged(P<0.05);Compared with the control,the first time of reaching platform of offspring in the high-fluoride group prolonged,and the frequency of crossing the platform reduced significantly(P<0.05);Compared with the control,the expression of GFAP in hippocampus of offspring in high-fluoride group significantly increased(P<0.05).Conclusion Maternal exposure to coal-burning fluoride can impair the offspring rats’learning and memory ability,the increased expression of GFAP in hippocampus of offspring may be involved in it.
作者
扈丽
王正蓉
喻茂娟
杨兰
王金玉
HU Li;WANG Zheng-rong;YU Mao-juan;YANG Lan;WANG Jin-yu(Department of Epidemiology,School of Public Health,Guizhou Medical University,Guiyang,Guizhou 550025,China)
出处
《环境与健康杂志》
CAS
北大核心
2019年第4期301-304,共4页
Journal of Environment and Health
基金
国家自然科学基金-贵州省人民政府联合基金(U1812403)
国家自然科学基金(81560511)
贵州省科技计划项目(黔科合LH字[2014]7127号)
关键词
燃煤型氟中毒
仔鼠
学习记忆
海马
GFAP
Coal-burning fluoride poisoning
Offspring
Learning and memory
Hippocampus
GFAP
