摘要
甲醛是广泛应用的病毒灭活剂,但其缺点也很明显,如刺激性强、破坏免疫原性、灭活不彻底等,因此需要寻找一种可以替代传统灭活剂甲醛的新型灭活剂。通过细胞盲传三代、无菌检验和台盼蓝染色等方法,结合不同灭活浓度与灭活时间双因素来筛选聚维酮碘与甲醛灭活牛传染性鼻气管炎病毒的最优条件,同时,向MDBK细胞中接种不同浓度的聚维酮碘和甲醛观察是否对MDBK细胞具有毒性和刺激性。结果表明10%的聚维酮碘24 h时可彻底灭活传染性鼻气管炎病毒,0.3%的甲醛48 h时可彻底灭活牛传染性鼻气管炎病毒,0.5%的甲醛对细胞具有强烈刺激导致绝大多数细胞死亡,而浓度为15%的聚维酮碘对MDBK细胞仍无刺激,直到浓度高达20%时才对细胞有了较大刺激,说明聚维酮碘无刺激性和毒性、安全性更高。为病毒灭活疫苗的理想灭活剂提供了新的方向,并为新型灭活剂的研发奠定基础。
Formaldehyde was a widely used virus inactivator,but its disadvantages were also obvious,such as strong irritant,destructive immunogenicity,incomplete inactivation,so it was urgent to look for a new type of formaldehyde which could replace the traditional formaldehyde inactivated.Through the blind passage of cells for three generations,sterility testing,and trypan blue staining,combining different inactivation concentration and inactivation time to screen the optimal conditions for povidone-iodine and formaldehyde inactivation of infectious rhinotracheitis virus.At the same time,inoculation of MDBK cells with various concentrations of povidone iodine and formaldehyde was observed to be toxic and irritant to MDBK cells.The results showed that 10%povidoneiodine completely inactivated infectious rhinotracheitis virus at 24 h,0.3%formaldehyde completely inactivated infectious rhinotracheitis virus at 48 h,and 0.5%formaldehyde strongly irritated cells resulting in the most dead cells,but 15%povidone-iodine had still no stimulation to MDBK cells,until the concentration of up to 20%of the cells had a greater stimulus,indicating povidone-iodine non-irritating,toxic and had more security.It provided a new direction for the ideal inactivators of virus inactivated vaccines,and laid the foundation for the development of new inactivators.
作者
辛志浩
刘明明
刘行波
倪宏波
Xin Zhihao;Liu Mingming;Liu Xingbo;Ni Hongbo(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319)
出处
《黑龙江八一农垦大学学报》
2019年第1期42-48,55,共8页
journal of heilongjiang bayi agricultural university
关键词
牛传染性鼻气管炎病毒
甲醛
聚维酮碘
灭活剂
灭活效果
bovine infectious rhinotracheitis virus
formaldehyde
povidone iodine
inactivating agent
inactivation effect
